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仲本 準 ナカモト ヒトシ

所属部署名 理工学研究科 生命科学部門 電話番号
職名 准教授 ■FAX番号
住所 埼玉県さいたま市桜区下大久保255 ■メールアドレス
■ホームページURL http://www.sci.saitama-u.ac.jp/molbiol/taisha/nakamoto.htm

プロフィール

兼担研究科・学部

理学部 分子生物学科 分子・細胞生理
環境科学研究センター

研究分野

光合成関連タンパク質及びストレスタンパク質(HSP、分子シャペロン)の生化学・分子生物学
ストレス応答の分子機構

現在の研究課題

ピルビン酸リン酸ジキナーゼなどの光合成(暗反応)酵素の活性調節機構を解明するために,無細胞系(Plant Physiol., Vol. 69, 1982;Biochem. Biophys. Res. Commun., Vol. 115, 1983;Biochim. Biophys. Acta, Vol. 924, 1987 ; Plant Physiol. Biochem., Vol. 28, 1990),葉緑体(Plant Cell Physiol., Vol. 31, 1990),細胞(Plant Physiol., Vol. 82, 1986),及び個葉レベル(Plant Physiol., Vol. 71, 1983 ; Aust. J. Plant Physiol., Vol. 10, 1983.)の研究を行い,総説も著わした(Ann. Rev. Plant Physiol., Vol. 36, 1985)。また,光合成酵素の細胞種あるいは葉齢特異的発現調節を解析した(Plant Physiol., Vol. 78, 1985 ; Plant Physiol., Vol. 92, 1990)。 シアノバクテリアの光合成(明反応)光化学系Ⅰ反応中心を構成するPsaD, PsaI, PasK及びPsaLの各サブユニットタンパク質遺伝子のクローニングとそれらの遺伝子破壊株の構築・表現型の解析(J. Biol. Chem., Vol. 268, 1993;Plant Cell Physiol., Vol. 36, 1995 ; Plant Cell Physiol., Vol. 40, 1999)を行った。 低分子量熱ショックタンパク質(HSP)やHtpG遺伝子をシアノバクテリアから初めてクローニングし(J. Bacteriol., Vol. 180, 1998;FEBS Lett., Vol. 458, 1999),HSPタンパク質の構造機能解析(Eur. J. Biochem., Vol. 262, 1999)と,遺伝子破壊株やHSP大量発現株を構築して高温などのストレス下における各HSPの生理的機能解析(FEBS Lett., Vol. 458, 1999 ; FEBS Lett., Vol. 483, 2000 ; Curr. Microbiol., Vol. 44, 2002; Curr. Microbiol., Vol. 46, 2003; FEBS Lett., Vol. 549, 2003; Arch. Microbiol. Vol.182, 2004; Curr. Microbiol., Vol. 50, 2005; FEBS Lett., Vol. 579, 2005)を行い,さらに植物のHSPに関する総説も著わした(Handbook of Plant and Crop Stress, 2nd Ed., 1999)。HSP遺伝子の発現調節機構に関しては、低分子量HSP遺伝子の転写調節に関与する新規なDNA配列(オペレーター配列)を発見し(Biochem. Biophys. Res. Commun. 297, 2002)、低分子量HSP遺伝子の転写後調節(Biochem. Biophys. Res. Commun. 331, 2005)、CIRCEオペレーター/HrcAリプレッサーによるgroEL遺伝子の転写調節(FEBS Lett., Vol. 549, 2003)、光によるシアノバクテリアHSP遺伝子発現調節(Biochem. Biophys. Res. Commun. 306, 2003)等の解析を行なってきた。データベース上に相同な配列が存在しない新規なHSP遺伝子(orf7.5)をクローニングして,これがgroESLオペロンの発現調節に関与していることを明らかにした(J. Biol. Chem., Vol. 276, 2001)。 東京農業大学吉川博文教授や東京大学田中寛助教授との共同研究により、このOrf7.5が主要シグマ因子と特異的に相互作用しgroELオペロンなどの転写を活性化することを明らかにした。今後、生化学的、分子遺伝学的及び分子形態学的(J. Bacteriol., 2006)方法を駆使して(光合成生物固有の)HSPの構造と機能を明らかにしたい。さらにシアノバクテリアHSP遺伝子の発現調節機構を解明し,生物におけるHSP遺伝子発現の多様性や普遍性に関する知見を得たい。

所属学会

所属学会
日本植物生理学会
日本分子生物学会
日本光合成学会
日本生物物理学会

学歴

出身大学院・研究科等
1984 , ワシントン州立大学 , 博士後期 , College of Sciences and Arts , 植物学 , 修了
取得学位
ワシントン州立大学 , Regulation of the activation of pyruvate, Pi dikinase and NADP-malate dehydrogenase in maize.
農学修士

受賞学術賞

1983 , American Society of Plant Physiologists International Travel Award

研究職歴等

研究職歴
1995 , 埼玉大学理学部助教授
1991 - 1991 , 日本学術振興会派遣研究員として米国ロシュ分子生物学研究所客員研究員
1989 - 1995 , 埼玉大学理学部講師
1987 - 1989 , 東京大学アイソトープ総合センター生物部門助手
1986 - 1987 , 農林水産省農業生物資源研究所研究員
1984 - 1986 , 米国ワシントン州立大学植物学科研究員
1984 - 1984 , スウェーデンウメオ大学植物生理学科客員研究員
1983 - 1984 , 英国シェフィールド大学光合成研究所研究員

研究活動業績

研究業績(著書・発表論文等)

著書
Handbook of Plant and Crop Stress. Third Edition.
CRC Press (Taylor & Francis Group):519-534 2010
Huq, S. and H. Nakamoto

論文
Comparative Biochemical Characterization of Two GroEL Homologs from the Cyanobacterium Synechococcus elongatus PCC 7942.
,Bioscience, Biotechnology, and Biochemistry,74(11):2273 - 2280 2010
Huq, S., Sueoka, K., Narumi, S., Arisaka, F. and H. Nakamoto

HtpG, the prokaryotic homolog of Hsp90, stabilizes a phycobilisome protein in the cyanobacterium Synechococcus elongatus PCC 7942.
,Molecular Microbiology,76(3):576 - 589 2010
Sato, T., Minagawa, S., Kojima, E., Okamoto, N. and H. Nakamoto

The middle domain of HtpG, a prokaryotic homolog of Hsp90, binds and stabilizes a phycobilisome linker polypeptide, an in vivo target in the cyanobacterium Synechococcus elongatus PCC 7942
,Mol. Microbiol. 2010
Sato, T., Minagawa, S., Kojima, E., Okamoto, N. and H. Nakamoto

シアノバクテリアの二種類のシャペロニン(GroEL)における機能分化
,CACS FORUM,1:33-36 2010
仲本準

A small heat-shock protein confers stress tolerance and stabilizes thylakoid membrane proteins in cyanobacteria under oxidative stress
Springer,Archives of Microbiology,191(4):319-328 200904
K. Sakthivel;T. Watanabe;H. Nakamoto

The NADPH thioredoxin reductase C functions as an electron donor to 2-Cys peroxiredoxin in a thermophilic cyanobacterium Thermosynechococcus elongatus BP-1.
Elsevier,Biochemical and Biophysical Research Communications,380(3):520-524 2009
Sueoka, K., Yamazaki, T., Hiyama, T. and H. Nakamoto

高等植物における熱ショック応答の分子機構と応用
社団法人 農林水産技術情報協会,農林水産技術 研究ジャーナル,32(7):5-9 2009
仲本準

Expression and function of a groEL paralog in the thermophilic cyanobacterium Thermosynechococcus elongatus under heat and cold stress
ELSEVIER SCIENCE BV,FEBS LETTERS,582(23-24):3389-3395 200810
Shinichiro Sato;Masahiko Ikeuchi;Hitoshi Nakamoto

Interaction of the molecular chaperone HtpG with uroporphyrinogen decarboxylase in the cyanobacterium Synechococcus elongatus PCC 7942
,Bioscience Biotechnology and Biochemistry,72(5):1394-1397 200805
Masakazu Saito;Satoru Watanabe;Hirofumi Yoshikawa;Hitoshi Nakamoto

Role of small heat shock protein and DNA-binding protein from starved cells, Dps, in oxidative stress management.
Research Trends,Current Topics in Biochemical Research,10(2):79-86 2008(
Sakthivel, K., Rai, L.C.and H.Nakamoto

A novel light- and heat-responsive regulation of the groE transcription in the absence of HrcA or CIRCE in cyanobacteria
,FEBS Lett.,581:1871-1880 2007
Kojima, K. and H. Nakamoto

Membrane regulation of the stress response from prokaryotic models to Mammalian cells (Invited review)
,Ann. N. Y. Acad. Sci.,1113:40-51 2007
Vigh, L., Nakamoto, H., Landry, J., Gomez-Munoz, A., Harwood, J.L. and I. Horvath

Studies on the role of HtpG in the tetrapyrrole biosynthesis pathway of the cyanobacterium Synechococcus elongatus PCC 7942
,Biochem. Biophys. Res. Commun.,352:36-41 2007
Watanabe, S., Kobayashi, T., Saito, M., Sato, M., Nimura-Matsune, K., Chibazakura, T., Taketani, S., Nakamoto, H. and H.Yoshikawa

The small heat shock proteins and their clients (Invited review)
,Cell. Mol. Life Sci.,64(3):294-306 2007
Nakamoto, H. and L. V gh

集光性超分子フィコピリソームの構築に関わる分子シャペロンの作用機構の解明
埼玉大学総合研究機構,総合研究機構研究プロジェクト研究成果報告書,5 (18年度):651-652 2007
仲本準
Functions of molecular chaperones in the assembly of the light-harvesting phycobilisomes

藻類によるCO2高効率固定にむけたCO2マイクロバブルの利用
埼玉大学総合研究機構地域共同研究センター産学連携推進部門,埼玉大学地域共同研究センター紀要,8:19-21 2007
仲本準,藤田健作,張凱
Cyanobacteria are photosynthetic eubacteria. Some cyanobacteria are used as a food source and as nutritional supplements. For commercial uses of cyanobacteria, it is important to find an efficient way to grow them. In the present study, we examined various factors that affect the efficiency of carbon dioxide fixation by cyanobacteria. A novel method to supply inorganic carbon source, that is, the supply of micro-bubbled carbon dioxide, is described.
Carbon dioxide, Carbon fixation, Cyanobacterium, Micro bubble, Nano bubble, Photosynthesis
Utilization of CO2 Micro Bubbles for High-efficiency CO2 Fixation by Cyanobacteria

Intact Carboxysomes in a cyanobacterial cell visualized by Hilbert differential contrast transmission electron microscopy
J. Bacteriol.,188(2):805-808 2006
Kaneko, Y., R. Danev, K. Nagayama and H. Nakamoto

Interaction of a small heat shock protein with light-harvesting cyanobacterial phycocyanins under stress conditions
,FEBS Lett.,580(13):3029-3034 2006
Nakamoto, H. and D. Honma

Light-driven hydrogen production by a hybrid complex of a [NiFe]-hydrogenase and the cyanobacterial photosystem I
,Photochem. Photobiol.,82(3):676-682 2006
Ihara M, Nishihara H, Yoon KS, Lenz O, Friedrich B, Nakamoto H, Kojima K, Honma D, Kamachi T, Okura I.

Photoinduced Hydrogen Production by Direct Electron Transfer from Photosystem I Cross-linked with Cytochrome c3 to [NiFe]-Hydrogenase
,Photochem. Photobiol.,82:1677-1686 2006
Ihara, M., H. Nakamoto, T. Kamachi, I. Okura and M. Maeda.

Roles of the cyanobacterial isiABC operon in protection from oxidative and heat stresses
,Physiol. Plant.,128:507-519 2006
Kojima, K., T. Suzuki-Maenaka, T. Kikuchi and H. Nakamoto

光合成装置の構築における分子シャペロンの役割
埼玉大学総合研究機構,総合研究機構研究プロジェクト研究成果報告書,4 (17年度) 2006
仲本準
The roles of molecular chaperones in the assembly of photosynthetic apparatus

Constitutive expression of small heat shock protein in an htpG disruptant of the cyanobacterium Synechococcus sp. PCC 7942
Curr. Microbiol.,50(5):272-276 2005
Kojima, K. and H. Nakamoto

Post-transcriptional control of the cyanobacterial hspA heat-shock induction
Biochem. Biophys. Res. Commun.,331(2):583-588 2005
Kojima K. and H. Nakamoto

Ultrastructural stability under high temperature or intensive light stress conferred by a small heat shock protein in cyanobacteria
FEBS Lett.,579(5) 2005
Nitta, K., N. Suzuki, D. Honma, Y. Kaneko and H. Nakamoto

パンもマーガリンも,増産の鍵はルビスコにある?光合成の主役ルビスコは脂肪酸合成にも駆りだされている
,蛋白質核酸酵素,50(5):450-451 2005
仲本準

Catalysis of disulfide bond formation and isomerization in the Escherichia coli periplasm. (Invited review).
Biochim. Biophys. Acta (Molecular Cell Research Special Issue),1694:111-119 2004
Nakamoto, H. and J. C. A. Bardwell

Comparative analysis of hspA mutant and wild type Synechocystis sp. PCC 6803 under salt stress: Evaluation of the role of hspA in salt stress management
Arch. Microbiol.,182(6):487-497 2004
Asadulghani, K. Nitta, Y. Kaneko, K. Kojima, H. Fukuzawa, H. Kosaka, and H. Nakamoto.

Conserved temperature-dependent expression of RNA-binding proteins in cyanobacteria with different temperature optima
FEMS Microbiol. Lett.,225:137-142 2003
Ehira, S., T. Hamano, T. Hayashida, K. Kojima, H. Nakamoto, T. Hiyama, M. Ohmori, S. Shivaji and N. Sato

Light plays a key role in the modulation of heat shock response in the cyanobacterium Synechocystis sp. PCC 6803
Biochem. Biophys. Res. Commun.,306:872-879 2003
Asadulghoni, Y. Suzuki, and H. Nakamoto.

Role of the cyanobacterial HtpG in protection from oxidative stress
Curr. Microbiol.,46:70-76 2003
Hossain, M. M. and H. Nakamoto.

Targeted inactivation of the hrcA repressor gene in cyanobacteria
FEBS Lett.,549:57-62 2003
Nakamoto, H., M. Suzuki and K. Kojima

HtpG plays a role in cold acclimation in cyanobacteria
Curr. Microbiol.,44:291-296 2002
Hossain, M. M. and H. Nakamoto

Specific binding of a protein to a novel DNA element in the cyanobacterial small heat-shock protein gene
Biochem. Biophys. Res. Commun.,297:616-624 2002
Kojima, K. and H. Nakamoto

A novel heat shock protein plays an important role for the thermal stress management in cyanobacteria
J. Biol. Chem.,276:25088-25095 2001
Nakamoto, H., N. Tanaka and N. Ishikawa

Heat shock proteins play a role in both high and low temperature stresses in cyanobacteria.
CSIRO Publishing,PS2001 Proceedings, 12th International Congress on Photosynthesis,S35-023:1-5 2001
Nakamoto, H., M.M. Hossain, N. Suzuki and K. Kojima.

Chromatographic separation of a small subunit (PsbW/PsaY) and its assignment to photosystem I reaction center
Biochim. Biophys. Acta (Bioenergetics),1459:117-124 2000
Hiyama, T., K. Yumoto, A. Satoh, M. Takahashi, T. Nishikido, H. Nakamoto, K. Suzuki and T. Hiraide.

Constitutive expression of a small heat-shock protein confers cellular thermotolerance and thermal protection to the photosynthetic apparatus in cyanobacteria
FEBS Lett.,483:169-174 2000
Nakamoto, H., N. Suzuki and S. K. Roy.

Targeted inactivation of the gene psaK encoding a subunit of photosystem I from the cyanobacterium Synechocystis sp. PCC 6803.
,Plant Cell Physiol.,40:9-16 1999
Nakamoto, H. and M. Hasegawa.

Heat Shock Proteins and Temperature Stress. (Invited review)
Marcel Dekker.,In: Pessarakli, M. ed., Handbook of Plant and Crop Stress. 2nd Ed.,Chapter 17:399-416 1999
Nakamoto, H. and T. Hiyama.

HtpG is essential for the thermal stress management in cyanobacteria.
,FEBS Lett.,458:117-123 1999
Tanaka, N. and H. Nakamoto

Purification and characterization of the 16-kDa heat-shock-responsive protein from the thermophilic cyanobacterium Synechococcus vulcanus, which is an α-crystallin-related, small heat shock protein
Eur. J. Biochem.,262:406-416 1999
Roy, S. K., T. Hiyama and H. Nakamoto

Cloning, characterization, and transcriptional analysis of a gene encoding an α-crystallin-related, small heat shock protein from the thermophilic cyanobacterium Synechococcus vulcanus.
,J. Bacteriol.,180:3997-4001 1998
Roy, S. K. and H. Nakamoto

Cloning, characterization, and functional analysis of groESL operon from thermophilic cyanobacterium Synechococcus vulcanus.
,Biochim. Biophys. Acta (Protein Structure and Molecular Enzymology),1343:335-348 1997
Tanaka,N., T. Hiyama and H. Nakamoto

Cloning, characterization, and transcriptional studies of ferredoxin genes from the mesophilic cyanobacterium Synechocystis sp. PCC 6803 and thermophilic cyanobacterium Synechococcus vulcanus.
,Physiol. Plant.,101:199-205 1997
Nakamoto, H. and T. Suzuki.

Cloning, characterization, and functional analysis of groEL-like gene from thermophilic cyanobacterium Synechococcus vulcanus, which does not form an operon with groES.
,Biochim. Biophys. Acta (Protein Structure and Molecular Enzymology),1294:106-110 1996
Furuki, M., N. Tanaka, T. Hiyama and H. Nakamoto

Some studies with mutant strains in which specific genes encoding subunits of photosystem I were inactivated
Kluwer Academic Publishers.,In: P. Mathis, ed., Photosynthesis: From Light to Biosphere.,3:513-516. 1996
Nakamoto, H

Nucleotide sequence of the psaD gene from the thermophilic cyanobacterium Synechococcus vulcanus.
,Photosynth. Res.,46:265-268 1995
Sue, S., K. Sugiya, M. Furuki, T. Shimizu, Y. Inoue, H. Nakamoto and T. Hiyama

Targeted inactivation of the gene psaI encoding a subunit of photosystem I of the cyanobacterium Synechocystis sp. PCC6803.
,Plant Cell Physiol.,36:1579-1587 1995
Nakamoto, H.

Targeted inactivation of the gene psaL encoding a subunit of photosystem I of the cyanobacterium synechocystis sp. PCC 6803.
,J. Biol. Chem.,268:11678-11684 1993
Chitnis, V. P., Q. Xu, L. Yu, J. H. Golbeck, H. Nakamoto, D. -L. Xie and P. R. Chitnis

Comparison of photosystem I complex isolated with different methods. In: N. Murata, ed., Research in Photosynthesis.
Kluwer Academic Publishers,In: N. Murata, ed., Research in Photosynthesis.,1:613-616 1992
Nakamoto, H and T. Hiyama.

The subunit stoichiometry of photosystem I reaction center.
Kluwer Academic Publishers,In: N. Murata, ed., Research in Photosynthesis.,1:621-624 1992
Hiyama, T., T. Oya, S. Kobayashi, M. Furuki, T. Shimizu, M. Senda and H. Nakamoto

Applications of capillary electrophoresis to redox proteins and nucleotides in photosynthetic system.
,Anal. Sci.,7:1545-1548 1991
Senda, M., T. Sasaki, H. Nakamoto and T. Hiyama

Changes in the rate of photosynthesis during grain filling and the enzymatic activities associated with the photosynthetic carbon metabolism in rice panicles.
,Plant Cell Physiol.,31:835-843 1990
Imaizumi, N., H. Usuda, H. Nakamoto and K. Ishihara

Effect of magnesium, manganese and phosphate on catalysis of pyruvate, orthophosphate dikinase from maize.
,Plant Physiol. Biochem.,28:553-559 1990
Nakamoto, H. and G. E. Edwards

Light activation of pyruvate, orthophosphate dikinase in maize mesophyll chloroplasts
,Plant Cell Physiol.,31:1-6 1990
Nakamoto, H. and S. -Y. Park

Regulation of expression of carbon-assimilating enzymes by nitrogen in maize leaf.
,Plant Physiol.,92:963-969. 1990
Sugiharto, B., K. Miyata, H. Nakamoto, H. Sasakawa and T. Sugiyama.

Regulation of pyruvate, orthophosphate dikinase from maize leaves. Magnesium-dependent dimer-tetramer interconversion.
Kluwer Academic Publishers,In: M. Baltscheffsky, ed., Current Research in Photosynthesis.,4:183-186 1990
Nakamoto, H

Purification and characterization of NAD malic enzyme from leaves of Eleusine coracana and Panicum dichotomiflorum.
,Plant Physiol.,89:316-324 1989
Murata, T., R. Ohsugi, M. Matsuoka and H. Nakamoto

Far-red stimulated long-lived luminescence from barley protoplasts.
,Plant Sci.,55:1-7 1988
Nakamoto, H., L. -G. Sundblad, P. Gardeström and E. Sundbom.

Effect of adenine nucleotides on the reaction catalyzed by pyruvate, orthophosphate dikinase in maize.
,Biochim. Biophys. Acta.,924:360-368 1987
Nakamoto, H. and G. E. Edwards

Influence of leaf age on photosynthesis, enzyme activity, and metabolite levels in wheat.
,Plant Physiol.,84:1244-1248 1987
Suzuki, S., H. Nakamoto, M. S. B. Ku and G. E. Edwards

Sudden changes in the rate of photosynthetic oxygen evolution and chlorophyll fluorescence in intact isolated chloroplasts: The role of orthophosphate.
,Photosynth. Res.,11:119-130 1987
Nakamoto, H., M. N. Sivak and D. A. Walker

Light activation of pyruvate, Pi dikinase and NADP-malate dehydrogenase in mesophyll protoplasts of maize: Effect of DCMU, Antimycin A, CCCP, and Phlorizin.
,Plant Physiol.,82:312-315 1986
Nakamoto, H. and G. E. Edwards.

Pyruvate, Pi dikinase and NADP-malate dehydrogenase in C4 photosynthesis: Properties and mechanism of light/dark regulation (Review)
,Ann. Rev. Plant Physiol.,36:255-286. 1985
Edwards, G. E., H. Nakamoto, J. N. Burnell and M. D. Hatch

Pyruvate, Pi dikinase in bundle sheath strands as well as in mesophyll cells in maize leaves.
,Plant Physiol.,78:661-664. 1985
Aoyagi, K. and H. Nakamoto

Dark activation of NADP-malate dehydrogenase in maize leaf discs.
,Z. Pflanzenphysiol.,114:315-320 1984
Nakamoto, H. and G. E. Edwards

Regulation of the activation of pyruvate, Pi dikinase and NADP-malate dehydrogenase in maize.
Martinus Nijhoff/Dr.W.Junk Publishers.,In: C. Sybesma, ed., Advances in Photosynthesis Research.,3:601-604 1984
Nakamoto, H. and G. E. Edwards

Control of the activation/inactivation of pyruvate, Pi dikinase from the C4 plant maize by adenylate energy charge, pyruvate, and analogs of pyruvate.
,Biochem. Biophys. Res. Commun.,115:673-679. 1983
Nakamoto, H. and G. E. Edwards

Dark activation of maize leaf NADP-malate dehydrogenase and pyruvate, orthophosphate dikinase in vivo under anaerobic conditions.
,Plant Sci. Lett.,32:139-146 1983
Nakamoto, H. and G. E. Edwards

Influence of environmental factors on the light activation of pyruvate, Pi dikinase and NADP-malate dehydrogenase in maize
,Aust. J. Plant Physiol.,10:279-289. 1983
Nakamoto, H. and G. E. Edwards

Influence of oxygen and temperature on the dark inactivation of pyruvate, orthophosphate dikinase and NADP-malate dehydrogenase in maize
,Plant Physiol.,71:568-573 1983
Nakamoto, H. and G. E. Edwards

Photosynthetic characteristics of C3-C4 intermediate Flaveria species. I. Leaf anatomy, photosynthetic responses to O2 and CO2, and activities of key enzymes in the C3 and C4 pathways
,Plant Physiol.,71:944-948 1983
Ku, M. S. B., R. K. Monson, R. O. Littlejohn, H. Nakamoto, D. B. Fisher and G. E. Edwards

Photosynthetic characteristics of C3-C4 intermediate Flaveria species. II. Kinetic properties of phospho-enolpyruvate carboxylase from C3, C4 and C3-C4 intermediate species.
,Plant Cell Physiol.,24:1387-1393 1983
Nakamoto, H., M. S. B. Ku and G. E. Edwards

Inhibition of C4 photosynthesis by (benzamidooxy)acetic acid.
,Photosynth. Res.,3:293-305 1982
Nakamoto, H., M. S. B. Ku and G. E. Edwards

Partial characterization of the in vitro activation of inactive pyruvate, Pi dikinase from darkened maize leaves
,Plant Physiol.,69:749-753 1982
Nakamoto, H. and T. Sugiyama.

学会発表
シアノバクテリアのHsp90/Hsp70シャペロンシステム
日本農芸化学会2011年度大会(京都女子大学、京都),大会講演要旨集:269 201103
仲本 準、藤田 健作

シアノバクテリアThermosynechococcus elongatus BP-1のNADPH thioredoxin reductase Cは2-Cys peroxiredoxinとNADPHによって制御される分子シャペロン活性を持つ
日本植物生理学会2011年度(第52回)年会(東北大学・川内北キャンパス、仙台) 201103
末岡 啓吾, 仲本 準

シアノバクテリアのHsp90とHsp70シャペロン系との協同的シャペロン作用
日本植物生理学会2011年度(第52回)年会(東北大学・川内北キャンパス、仙台) 201103
仲本 準、藤田健作

Modulation of the Hsp90 function by small molecules
International Conference on Natural Products and Biomedical technology (Annamalai University, India),International Conference on Natural Products and Biomedical technology ICNPBT2011 201101
仲本準

Prokaryotic Hsp90: its substrates, chaperone sites, chaperone function, and collaboration with the Hsp70 system
The 5th International Conference on the Hsp90 Chaperone Machine (Les Diablerets, Switzerland),The 5th International Conference on the Hsp90 Chaperone Machine:112 201009
仲本準

原核生物Hsp90の機能
第48回日本生物物理学会年会(東北大学・川内北キャンパス、仙台),生物物理Supplement2,50:S153 201009
仲本準、 藤田健作、 星野秀知

ラン藻Hsp90の機能
ラン藻ゲノム研究交流会(東京大学・駒場キャンパス、駒場) 201007
仲本準

シアノバクテリアGroELの生化学的解析
第9回 微生物研究会 「微生物分子生物学の新たな挑戦」(法政大学、小金井) 201006
末岡啓吾、ホック・サイマトゥル、仲本準

シアノバクテリアHsp90のシャペロン部位と機能
第9回 微生物研究会 「微生物分子生物学の新たな挑戦」(法政大学、小金井) 201006
星野秀知、仲本準

シアノバクテリアの多機能ストレスタンパク質NTRC
第9回 微生物研究会 「微生物分子生物学の新たな挑戦」(法政大学、小金井) 201006
末岡啓吾、仲本準

Function of prokaryotic Hsp90, a forgotten chaperone
The 8th International Workshop on the Molecular Biology of Stress Responses (Daemyung Resort Mt., Korea),8th International Workshop on the Molecular Biology of Stress Responses:18 201006
仲本準

シアノバクテリアの二種類のGroEL(シャペロニン):タンパク質の構造、活性、細胞機能
日本植物生理学会2010年度(第51回)年会,第51回日本植物生理学会年会要旨集:294 201003
仲本準、Saaimatul Huq、末岡啓吾、有坂文雄
熊本大学黒髪北キャンパス、熊本

Cyanobacterial diversity and molecular chaperones
International Symposium on Phycological Research (Varanasi, India),International Symposium on Phycological Research Abstract:18 201002
仲本準

Chemical array-based screening of inhibitors for cyanobacterial HtpG (an eukaryotic Hsp90 homologue)
The 5th Japan- Korea Chemical Biology Symposium (Busan, Korea) 201001
Yasumitsu Kondoh, Shun Minagawa, Akiko Saito, Hitoshi Nakamoto, Hiroyuki Osada

Chaperone activity of an NADPH thioredoxin reductase C from the cyanobacterium Thermosynechococcus elongatus
第32回日本分子生物学会年会,第32回日本分子生物学会年会(パシフィコ横浜、横浜):371 200912
末岡啓吾、仲本準

Cyanobacterial DnaK/DnaJ/GrpE chaperone system and its control by HtpG (Hsp90)
第32回日本分子生物学会年会,第32回日本分子生物学会年会(パシフィコ横浜、横浜):371 200912
藤田健作、仲本準

NADPHチオレドキシンレダクターゼCの分子シャペロン活性
かずさDNA研究所研究会 ラン藻の分子生物学2009,かずさDNA研究所研究会 ラン藻の分子生物学2009:37 200912
末岡啓吾、仲本準
かずさアカデミアホール、木更津

シアノバクテリアの環境適応と分子シャペロン
かずさDNA研究所研究会,ラン藻の分子生物学2009:18 200912
仲本準
かずさアカデミアホール、木更津

シアノバクテリアDnaK/DnaJ/GrpE系の確立とHtpGによるリフォ ルディング活性の調節
第47回日本生物物理学会年会,生物物理Supplement 1「第47回年会講演予稿集」,49 200910
藤田健作、仲本準(仲本が発表した)
アスティとくしま、徳島

Functional specialization of two different GroEL homologues in cyanobacteria
The 4th International Congress on Stress Responses in Biology and Medicine & the 4th Annual Meeting of the Biomedical Society for Stress Response,The 4th International Congress on Stress Responses in Biology and Medicine & the 4th Annual Meeting of the Biomedical Society for Stress Response (Sapporo, Japan):71 200910
仲本準
Sapporo, Japan

Response to environmental stresses in cyanobacteria: Role of molecular chaperones
Microbial response to environmental stress: Molecular mechanisms and applications 200910
仲本準
埼玉大学

河川の礫付着藻類の蛍光測定と評価の検討
日本陸水学会,日本陸水学会第74回大会 200909
Tabucanon Allan、藤野毅、仲本準

シアノバクテリアDnaK/DnaJ/GrpEシステムの確立とシャペロン活性に及ぼすHtpG(Hsp90)の影響
特定領域研究「生体超分子構造」第5回ワークショップ 200907
仲本準、藤田健作
湘南国際村センター国際会議場

シアノバクテリアDnaK/DnaJ/GrpEシステム
ラン藻ゲノム交流会 200907
仲本準
東京大学駒場キャンパスI、東京

酸化ストレスで誘導される、シアノバクテリアNADPH thioredoxin reductase C / 2-Cys peroxiredoxin複合体の生化学的解析
日本光合成研究会,第9回公開シンポジウム「植物の生産性を支える多様な側面」 200905
末岡啓吾、山崎映明、檜山哲夫、仲本準
東京大学駒場キャンパスI、東京

Stress response and chaperone function in cyanobacteria
The EMBO Coference Series on: The Biology of Molecular Chaperones (Dubrovnik, Croatia),The Biology of Molecular Chaperones. Cellular Protein Homeostasis in Disease and Aging.:148 200905
仲本準

教育活動実績

授業等

STEP(理学部)
酵素学概論(理学部)
細胞生理学(理学部)
タンパク質の分子生物学(理学部)
基礎生物学実験(理学部)
代謝学実験(理学部)
代謝学演習(理学部)
前期 , 代謝学特論(院・理工学前期)
前期 , 代謝学輪講Ⅰ(院・理工学前期)
前期 , 代謝学輪講Ⅱ(院・理工学前期)
後期 , 植物分子生理学(院・理工学後期)

社会活動実績

社会活動等

学会・研究会の主催等
2009-2009,4th International Congress on Stress Responses in Biology and Medicine , シンポジウム企画
2009-2009 , 埼玉大学環境科学研究センター,国際シンポジウム , 主催