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日原　由香子 ヒハラ ユカコ

■所属部署名 理工学研究科　生命科学部門 ■電話番号
■職名 教授  科学分析支援センター長 ■FAX番号
■住所 埼玉県さいたま市桜区下大久保255 ■メールアドレス
■ホームページURL http://park.saitama-u.ac.jp/~idenshi/index.html

プロフィール

兼担研究科・学部

理学部　分子生物学科　分子・細胞生理
科学分析支援センター

研究分野

植物生理
分子生物学

現在の研究課題

光環境の変動に対し，光合成生物がどのように自らの光合成系を変化させて順化応答を示すか（Curr. Top. Plant Biol. Vol.１, 1999），その仕組みの遺伝子レベルでの解明を目指している。具体的には，遺伝子操作の容易なシアノバクテリアSynechocystis sp. PCC 6803から，強光下での光化学系２，系１複合体の量比調節に必須な遺伝子pmgAを初めて単離し，その機能解析を行った（photosynth. Res. Vol. 53, 1997；Plant Physiol. Vol. 117, 1998）。また，Synechocystis sp. PCC6803の全遺伝子を網羅したDNAマイクロアレイを用いて，強光順化時の遺伝子発現を経時的に調べた（Plant Cell Vol. 13, 2001）。今後はそれらの遺伝子発現の調節メカニズムを解析して行きたいと考えている。

所属学会

所属学会
日本植物学会
日本植物生理学会
The International Society of Photosynthesis Research

学歴

出身大学院・研究科等
1998 , 東京大学 , 博士 , 理学系研究科 , 修了
1995 , 東京大学 , 修士 , 理学系研究科 , 修了
出身学校・専攻等(大学院を除く)
1993 , 東京大学 , 理学部 , 卒業
取得学位
博士（理学） , 東京大学 , シアノバクテリアの強光順化に関わる遺伝子の単離とその生理学的解析

研究職歴等

研究職歴
2000 , 埼玉大学理学部助手
1997 - 2000 , 日本学術振興会特別研究員

研究活動業績

研究業績(著書・発表論文等)

著書
‘Biosynthesis of Fatty Acid Derivatives by Cyanobacteria: From Basics to Biofuel Production’ in "Cyanobacteria Biotechnology"
Wiley:331-368
Kawahara A, Hihara Y

Plastid Development in Leaves during Growth and Senescence
Springer:101-116
Hihara Y, Sonoike K

論文
The NAD kinase Slr0400 functions as a growth repressor in Synechocystis sp. PCC 6803
Plant Cell Physiology,62(4): 668–677 2021
Ishikawa Y. et al.

シアノバクテリアの転写因子と物質生産
生物工学会誌 ,99 (8):416-420 2021
日原由香子

The role of transcriptional repressor activity of LexA in salt-stress responses of the cyanobacterium Synechocystis sp. PCC 6803.
Scientific Reports ,10:17393 2020
Takashima K., Nagao S., Kizawa A., Suzuki T., Dohmae N. and Hihara Y.

Quantitative and qualitative analyses of triacylglycerol production in the wild-type cyanobacterium Synechocystis sp. PCC 6803 and the strain expressing AtfA from Acinetobacter baylyi ADP1.
Plant Cell Physiology,61:1537-1547 2020
Tanaka M., Ishikawa T., Tamura S., Saito Y., Kawai-Yamada M. and Hihara Y.

One of the NAD kinases, sll1415, is required for the glucose metabolism of Synechocystis sp. PCC 6803.
Plant Journal ,98:654-666 2019
Ishikawa Y., Miyagi A., Ishikawa T., Nagano M., Yamaguchi M., Hihara Y., Kaneko Y. and Kawai-Yamada M.

Biocomputational analyses and experimental validation identify the complex regulon controlled by the redox-responsive transcription factor RpaB.
iScience,15:316-331 2019
Riediger M., Kadowaki T., Nagayama R., Georg J., Hihara Y. and Hess W.R.

cyAbrB Transcriptional regulators as safety devices to inhibit heterocyst differentiation in Anabaena sp. Strain PCC 7120.
Journal of Bacteriology,201:e00244-19 2019
Higo A., Nishiyama E., Nakamura K., Hihara Y. and Ehira S.

Light-inducible expression of translation factor EF-Tu during acclimation to strong light enhances the repair of photosystem II.
Proc Natl Acad Sci U S A. ,116:21268-21273 2019
Jimbo H., Izuhara T., Hihara Y., Hisabori T. and Nishiyama Y.

Oxidation of translation factor EF-Tu inhibits the repair of photosystem II
Plant Physiology,176(4):2691-2699 2018
Jimbo H, Yutthanasirikul R, Nagano T, Hisabori T, Hihara Y, Nishiyama Y

From cyanobacteria and algae to land plants: The RpaB/Ycf27 regulatory network in transition.
Perspectives in Phycology,5(1):13-25 2018
Riediger M, Hihara Y, Hess WR

Interaction of the GntR-family transcription factor Sll1961 with thioredoxin in the cyanobacterium Synechocystis sp. PCC 6803.
Scientfic Reports ,8:6666 2018
Kujirai J, Nanba S, Kadowaki T, Oka Y, Nishiyama Y, Hayashi Y, Arai M, Hihara Y

Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803.
Biotechnology and Bioengineering,115(12):2974-2985 2018
Kodama Y, Kawahara A, Miyagi A, Ishikawa T, Kawai-Yamada M, Kaneko Y, Takimura Y, Hihara Y

The LexA transcription factor regulates fatty acid biosynthetic genes in the cyanobacterium Synechocystis sp. PCC 6803.
The Plant Journal,92 (2):189-198 2017
Kizawa A, Kawahara A, Takashima K, Takimura Y, Nishiyama Y, Hihara Y.

Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1.
Current Biology,27(10):1425-1436 2017
Georg J, Kostova G, Vuorijoki L, Schön V, Kadowaki T, Huokko T, Baumgartner D, Müller M, Klähn S, Allahverdiyeva Y, Hihara Y, Futschik ME, Aro EM, Hess WR.

Applying a riboregulator as a new chromosomal gene regulation tool for higher glycogen production in Synechocystis sp. PCC 6803.
Applied Microbiology and Biotechnology,101 (23-24):8465-8474 2017
Ueno K, Sakai Y, Shono C, Sakamoto I, Tsukakoshi K, Hihara Y, Sode K, Ikebukuro K.

多彩な戦略で挑むシアノバクテリア由来の持続可能燃料生産
化学と生物・日本農芸化学会,55:88-97 2017
日原由香子、朝山宗彦、蘆田弘樹、天尾豊、新井宗仁、粟井光一郎、得平茂樹、小山内崇、鞆達也、成川礼、蓮沼誠久、増川一

Transcriptional and posttranscriptional regulation of cyanobacterial photosynthesis
Biochimica et Biophysica Acta,1857 (3):296-308 2016
Wilde A, Hihara Y

RNA-seq profiling reveals novel target genes of LexA in the cyanobacterium Synechocystis sp. PCC 6803
Frontiers in Microbiology,7:193 2016
Kizawa A, Kawahara A, Takimura Y, Nishiyama Y, Hihara Y

A feed-forward loop consisting of the response regulator RpaB and the small RNA PsrR1 controls light acclimation of photosystem I gene expression in the cyanobacterium Synechocystis sp. PCC 6803
Plant and Cell Physiology,57 (4):813-823 2016
Kadowaki T, Nagayama R, Georg J, Nishiyama Y, Wilde A, Hess WR, Hihara Y

Free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803 is enhanced by deletion of the cyAbrB2 transcriptional regulator
Journal of Biotechnology,220:1-11 2016
Kawahara A, Sato Y, Saito Y, Kaneko Y, Takimura Y, Hagihara H, Hihara Y

Oxidation of a cysteine residue in elongation factor EF-Tu reversibly inhibits translation in the cyanobacterium Synechocystis sp. PCC 6803
Journal of Biological Chemistry,291:5860-5870 2016
Yutthanasirikul R, Nagano T, Jimbo H, Hihara Y, Kanamori T, Ueda T, Haruyama T, Konno H, Yoshida K, Hisabori T, Nishiyama Y

Metabolomic analysis of NAD kinase-deficient mutants of the cyanobacterium Synechocystis sp. PCC 6803
Journal of Plant Physiology,205:105-112 2016
Ishikawa Y, Miyagi A, Haishima Y, Ishikawa T, Nagano M, Yamaguchi M, Hihara Y, Kawai-Yamada M

Overexpressed superoxide dismutase and catalase act synergistically to protect the repair of PSII during photoinhibition in Synechococcus elongatus PCC 7942
Plant and Cell Physiology,57:1899-1907 2016
Sae-Tang P, Hihara Y, Yumoto I, Orikasa Y, Okuyama H, Nishiyama Y

Intracellular imaging of Qdots-labeled DNA in cyanobacteria
Microscopy Research and Technique,79:447-452 2016
Loukanov A, Zhelyazkov V, Hihara Y, Nakabayashi S

Moderate heat stress stimulates repair of photosystem II during photoinhibition in Synechocystis sp. PCC 6803
Plant and Cell Physiology,57:2417-2426 2016
Ueno M, Sae-Tang P, Kusama Y, Hihara Y, Matsuda M, Hasunuma T, Nishiyama Y

Zeaxanthin and Echinenone Protect the Repair of Photosystem II from Inhibition by Singlet Oxygen in Synechocystis sp. PCC 6803.
Plant Cell Physiol.,56:906 2015
Kusama Y, Inoue S, Jimbo H, Takaichi S, Sonoike K, Hihara Y, Nishiyama Y.

Identification of OmpR-family response regulators interacting with thioredoxin in the Cyanobacterium Synechocystis sp. PCC 6803.
PLoS One,10:e0119107 2015
Kadowaki T, Nishiyama Y, Hisabori T, Hihara Y.

Analysis of spontaneous suppressor mutants from the photomixotrophically grown pmgA-disrupted mutant in the cyanobacterium Synechocystis sp. PCC 6803.
Photosynth Res.,126:465 2015
Nishijima Y, Kanesaki Y, Yoshikawa H, Ogawa T, Sonoike K, Nishiyama Y, Hihara Y.

The Effects of Dark Incubation on Cellular Metabolism of the Wild Type Cyanobacterium Synechocystis sp. PCC 6803 and a Mutant Lacking the Transcriptional Regulator cyAbrB2.
Life (Basel),4:770-787 2014
Hanai M, Sato Y, Miyagi A, Kawai-Yamada M, Tanaka K, Kaneko Y, Nishiyama Y, Hihara Y.

Deletion of the transcriptional regulator cyAbrB2 deregulates primary carbon metabolism in Synechocystis sp. PCC 6803.
Plant Physiology,162:1153-1163 2013
Kaniya Y, Kizawa A, Miyagi A, Kawai-Yamada M, Uchimiya H, Kaneko Y,　Nishiyama Y, Hihara Y.

Acclimation to high-light conditions in cyanobacteria: from gene expression to physiological responses.
Journal of Plant Research ,125:11-39 2012
Muramatsu M, Hihara Y.

Physiological roles of the pair of cyAbrB transcriptional regulators, Sll0822 and Sll0359, in Synechocystis sp. strain PCC 6803.
Journal of Bacteriology ,193:3702-3709 2011
Yamauchi Y, Kaniya Y, Kaneko Y and Hihara Y

Cross-talk between photomixotrophic growth and CO2-concentrating mechanism in Synechocystis sp. strain PCC 6803.
Environmental Microbiology ,13:1767-1777 2011
Haimovich-Dayan M, Kahlon S, Hihara Y, Hagemann M, Ogawa T, Ohad I, Lieman-Hurwitz J, Kaplan A.

Role of multiple HLR1 sequences in the regulation of the dual promoters of the psaAB genes in Synechocystis sp. PCC 6803.
Journal of Bacteriology,192:4031-4036 2010
Takahashi T, Nakai N, Muramatsu M, Hihara Y

The PedR transcriptional regulator interacts with thioredoxin to connect photosynthesis with gene expression in cyanobacteria.
Biochemical Journal,431:135-140 2010
Horiuchi M, Nakamura K, Kojima K, Nishiyama Y, Hatakeyama W, Hisabori T, Hihara Y

Mechanism of downregulation of photosystem I content under high-light conditions in the cyanobacterium Synechocystis sp PCC 6803
Microbiology-Sgm,155:989-996 200903
M. Muramatsu;K. Sonoike;Y. Hihara
電子ジャーナル PubMed リポジトリ

The Response Regulator RpaB Binds to the Upstream Element of Photosystem I Genes To Work for Positive Regulation under Low-Light Conditions in Synechocystis sp Strain PCC 6803
Journal of Bacteriology,191(5):1581-1586 200903
Y. Seino;T. Takahashi;Y. Hihara
電子ジャーナル PubMed リポジトリ

A cyanobacterial AbrB-like protein affects the apparent photosynthetic affinity for CO2 by modulating low-CO2-induced gene expression.
Blackwell,Environmental Microbiology,11(4):927-936 2009
Lieman-Hurwitz J, Haimovich M, Shalev-Malul G, Ishii A, Hihara Y, Gaathon A, Lebendiker M and Kaplan A

Mechanism of downregulation of photosystem I content under high-light conditions in the cyanobacterium Synechocystis sp. PCC 6803.
Society for General Microbiology,Microbiology,155:989-996 2009
Muramatsu M, Sonoike K and Hihara Y

The response regulator RpaB binds to the upstream element of photosystem I genes to work for positive regulation under low-light conditions in Synechocystis sp. Strain PCC 6803.
American Society for Microbiology,Journal of Bacteriology,191(5):1581-1586 2009
Seino Y, Takahashi T and Hihara Y

シアノバクテリア光化学系Ⅰサブユニット遺伝子群の統一的な光応答機構の解明
埼玉大学総合研究機構,総合研究機構研究プロジェクト成果発表会 : ポスター展示 200811
日原 由香子
リポジトリ

An AbrB-like transcriptional regulator, Sll0822, is essential for the activation of nitrogen-regulated genes in Synechocystis sp PCC 6803
Plant Physiology,148(1):660-670 200809
Ai Ishii;Yukako Hihara
電子ジャーナル Web Of Science

シアノバクテリア光化学系Ⅰサブユニット遺伝子群の統一的な光応答機構の解明
埼玉大学総合研究機構,総合研究機構研究プロジェクト研究成果報告書(6(平成19年度)) 200809
日原 由香子
リポジトリ

Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry
Oxford University Press,JOURNAL OF EXPERIMENTAL BOTANY,59(11):3009-3018 200808
日原 由香子
電子ジャーナル Web Of Science リポジトリ

シアノバクテリアにおけるクロロフィル合成依存的な転写調節に関する研究
埼玉大学総合研究機構,総合研究機構研究プロジェクト研究成果報告書(5(18年度)):659-659 2007
日原 由香子
Transcriptional regulation dependent on chlorophyll biosynthesis in cyanobacteria
リポジトリ

Characterization of high-light-responsive promoters of the psaAB genes in Synechocystis sp PCC 6803
Oxford Univ. Press,Plant and cell physiology,47(7):878-890 2006
日原 由香子
リポジトリ

Photon flux density-dependent gene expression in Synechocystis sp PCC 6803 is regulated by a small, redox-responsive, LuxR-type regulator
American Society for Biochemistry and Molecular Biology,Journal of biological chemistry,281(48):36758-36766 2006
日原 由香子
リポジトリ

PsaK2 subunit in photosystem I is involved in state transition under high light condition in the cyanobacterium Synechocystis sp. PCC 6803.
American Society for Biochemistry and Molecular Biology,Journal of biological chemistry,280(23):22191-22197 2005
日原 由香子
リポジトリ

The mutant of sll1961, which encodes a putative transcriptional regulator, has a defect in regulation of photosystem stoichiometry in the cyanobacterium Synechocystis sp PCC 6803
American Society of Plant Biologists,Plant physiology,139(1):408-416 2005
日原 由香子
リポジトリ

A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions
Elsevier,FEBS letters,574:101-105 2004
日原 由香子
リポジトリ

DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp strain PCC 6803
American Society for Microbiology,Journal of bacteriology,185(5) 2003
日原 由香子
リポジトリ

Transcriptional regulation of genes encoding subunits of photosystem I during acclimation to high-light conditions in Synechocystis sp PCC 6803
Springer,Planta,216(3) 2003
日原 由香子
リポジトリ

DNA microarray analysis of cyanobacterial gene expression during acclimation to high light.
Plant Cell,13:793-806 2001

The molecular mechanism for acclimation to high light in cyanobacteria.
Current Topics in Plant Biology,1:37-50 1999

A novel gene, pmgA, specifically regulates photosystem stoichiometry in the cyanobacterium Synechocystis sp. PCC 6803 in response to high light.
Plant Physiology,117:1205-1216 1998

Toward the elucidation of physiological significance of pmgA-mediated high-light acclimation to adjust photosystem stoichiometry：Effects of the prolonged high-light treatment on pmgA mutants.
Photosynthesis：Mechanism and Effects:2929-2932 1998

Mutation in a novel gene required for photomixotrophic growth leads to enhanced photoautotrophic growth of Synechocystis sp. PCC 6803.
Photosynthesis Research,53(2/3):243-252 1997

学会発表
Physiological role of AbrB-type transcriptional regulators in cyanobacteria
日本フィンランド二国間セミナｰ,Future prospects of photosynthetic organisms: from genomes to environment:21 201103
Yukako Hihara

Light acclimation and transcriptional regulation in cyanobacteria
スイス日本二国間セミナｰ 201101
Yukako Hihara

転写因子CyAbrBと細胞内C/Nバランス制御
ラン藻ゲノム研究交流会 201007
日原由香子

シアノバクテリアにおけるAbrB型転写制御因子の機能解析
第4回日本ゲノム微生物学会年会,第4回日本ゲノム微生物学会年会 要旨集:59 201003
山内優輝、石井愛、日原由香子

Synechocystis sp. PCC 6803の光化学系Iサブユニット遺伝子psaABプロモーター構造の再検討
ラン藻の分子生物学2009,ラン藻の分子生物学2009 要旨集:19 200912
日原由香子

転写因子cyAbrB
ラン藻ゲノム研究交流会 200907
日原由香子

Transcriptional regulators working on environmental responses in cyanobacteria
第5回日独二国間セミナー "From Photoreaction to Biomass: Phototrophs in Ecosystems and Biotechnology",The 5th German-Japan Binational Seminar From Photoreaction to Biomass: Phototrophs in Ecosystems and Biotechnology:30 200906
Yukako Hihara

その他
ゲノムの研究の現在 : 埼玉から世界ヘ
埼玉大学, 読売新聞,埼玉大学創立60周年記念連続市民講座「埼玉学のすすめ : 埼玉の過去・現在・未来を知る」(第八回) 200910
日原 由香子
リポジトリ

教育活動実績

授業等

基礎生物学実験(理学部)
代謝学実験(理学部)
代謝学演習(理学部)
細胞生理学(理学部)