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Faculty: Graduate School of Science and Engineering TEL:
Position: Assistant Professor ■FAX:
Address: 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, JAPAN ■Mail Address:
■Web site:


Assigned Class

School of Engineering
Advanced Institute of Innovative Technology

Research Field

Synthetic chemistry
Polymer chemistry
Analytical chemistry
keyword:Carbohydrate , Peptide , Glycopeptide , Glycosyltransferase , Antibody , Synthetic polymer , Multivalent polymer , Antibiotics , Lectin

Academic Societies

Academic Societies
The Japanese Society of Carbohydrate Research , Regular member
The Chemical Society of Japan , Regular member
The Society of Polymer Science, Japan , Regular member

Academic Background

Graduate School
Hokkaido University , (Doctor later , Graduate School of Science , Division of Biological Sciences) , 2005(Accomplished credits for doctoral program)
Hokkaido University , (Doctor prophase , Graduate School of Science , Division of Biological Sciences) , 2002(Completed)
Hokkaido University , (Division of Biological Sciences , School of Science) , 2000(Graduated)
Ph.D. in science , Hokkaido University


2011 , Award for Encouragement of Research in Polymer Science , Chemical and Enzymatic Synthesis of MUC1 Glycopeptide Library

Research Career

Research Career
2015 , Saitama university, Assistant professor
2013 - 2015 , Research Scholar at Department of Chemistry, Wayne State University, USA
2008 - 2013 , Specially Appointed Assistant Professor at Faculty of Advanced Life Science, Hokkaido University, Japan
2005 - 2008 , Research Associate at National Institute of Advanced Industrial Science and Technology


Books, Articles, etc.

Methods in Enzymology vol.478 " Novel Synthesis of Functional Mucin Glycopeptides Containing Both N- and O-glycans."
Elsevier Inc.:485-502 201011
Takahiko Matsushita, Shin-Ichiro Nishimura

A constraint scaffold enhances affinity of a bivalent N-acetylglucosamine ligand against wheat germ agglutinin
,Bioorganic & Medicinal Chemistry Letters:in press 2018
Takahiko Matsushita, Koji Tsuchibuchi, Tetsuo Koyama, Ken Hatano, Koji Matsuoka

Iodoacetyl-functionalized pullulan: A supplemental enhancer for single-domain antibody–polyclonal antibody sandwich enzyme-linked immunosorbent assay for detection of survivin
,Bioorganic & Medicinal Chemistry Letters,27(21):4844-4848 2017
Takahiko Matsushita, Hidenao Arai, Tetsuo Koyama, Ken Hatano, Naoto Nemoto, Koji Matsuoka

The Quest for Anticancer Vaccines: Deciphering the Fine-Epitope Specificity of Cancer-Related Monoclonal Antibodies by Combining Microarray Screening and Saturation Transfer Difference NMR
,Journal of the American Chemical Society,137(39):12438-12441 2015
Helena Coelho, Takahiko Matsushita, Gerard Artigas, Hiroshi Hinou, F. Javier Cañada, Richard Lo-Man, Claude Leclerc, Eurico J. Cabrita, Jesús Jiménez-Barbero, Shin-Ichiro Nishimura, Fayna Garcia-Martín, Filipa Marcelo

Influence of 4′-O-Glycoside Constitution and Configuration on Ribosomal Selectivity of Paromomycin
,Journal of the American Chemical Society,137(24):7706-7717 2015
Takahiko Matsushita, Weiwei Chen, Reda Juskeviciene, Youjin Teo, Dimitri Shcherbakov, Andrea Vasella, Erik C. Böttger, David Crich

Fluorine-Decoupled Carbon Spectroscopy for the Determination of Configuration at Fully Substituted, Trifluoromethyl- and Perfluoroalkyl-Bearing Carbons: Comparison with 19F–1H Heteronuclear Overhauser Effect Spectroscopy
,The Journal of Organic Chemistry,80(3):1754-1763 2015
Appi Reddy Mandhapati, Takayuki Kato, Takahiko Matsushita, Bashar Ksebati, Andrea Vasella, Erik C. Böttger, David Crich

Fast epitope mapping for the anti-MUC1 monoclonal antibody by combining one-bead-one-glycopeptide library and microarray platform
,Chemistry - A European Journal,20(48):15891-15902 2014
Fayna Garcia-Martin, Takahiko Matsushita, Hiroshi Hinou, Shin-Ichiro Nishimura

Delineating Binding Modes of Gal/GalNAc and Structural Elements of the Molecular Recognition of Tumor-Associated Mucin Glycopeptides by the Human Macrophage Galactose-Type Lectin
,Chemistry - A European Journal,20(49):16147-16155 2014
Filipa Marcelo, Fayna Garcia-Martin, Takahiko Matsushita, João Sardinha, Helena Coelho, Anneloes Oude-Vrielink, Christiane Koller, Sabine André, Eurico J. Cabrita, Hans-Joachim Gabius, Shin-Ichiro Nishimura, Jesús Jiménez-Barbero, F. Javier Cañada

Synthesis, antiribosomal and antibacterial activity of 4′-O-glycopyranosyl paromomycin aminoglycoside antibiotics
,MedChemComm,5:1179-1187 2014
Weiwei Chen, Takahiko Matsushita, Dimitri Shcherbakov, Heithem Boukari, Andrea Vasella, Erik C. Böttger, David Crich

A straightforward protocol for the preparation of high performance microarray displaying synthetic MUC1 glycopeptides
,Biochimica Et Biophysica Acta-general Subjects,1840(3):1105-1116 2014
Takahiko Matsushita, Wataru Takada, Kota Igarashi, Kentaro Naruchi, Risho Miyoshi, Fayna Garcia-Martin, Maho Amano, Hiroshi Hinou, Shin-Ichiro Nishimura

Site-specific Conformational Alteration Induced by Sialylation of MUC1 Tandem Repeating Glycopeptides at An Epitope Region for Anti-KL-6 Monoclonal Antibody
,Biochemistry,52(2):402-414 2013
Takahiko Matsushita, Naoki Ohyabu, Naoki Fujitani, Kentaro Naruchi, Hiroki Shimizu, Hiroshi Hinou, Shin-Ichiro Nishimura

A Novel Approach for the Parallel Synthesis of Glycopeptides by Combining Solid-Phase Peptide Synthesis and Dendrimer-supported Enzymatic Modifications
,Polymer Journal,45(8):854-862 2013
Takahiko Matsushita, Seiji Handa, Kentaro Naruchi, Fayna Garcia-Martin, Hiroshi Hinou, Shin-Ichiro Nishimura

Microwave-Assisted Solid-Phase Synthesis of Antifreeze Glycopeptides
,Chemistry - A European Journal,19(12):3913-3920 2013
Ryuko Izumi, Takahiko Matsushita, Naoki Fujitani, Kentaro Naruchi, Hiroki Shimizu, Sakae Tsuda, Hiroshi Hinou, Shin-Ichiro Nishimura

Macrocyclic Mechanism-Based Inhibitor for Neuraminidases
,Chemistry - A European Journal,19(4):1364-1372 2013
Hirokazu Kai, Hiroshi Hinou, Kentaro Naruchi, Takahiko Matsushita, Shin-Ichiro Nishimura

An Efficient Protocol for the Solid-Phase Synthesis of Glycopeptides under Microwave Irradiation
,Organic & Biomolecular Chemistry,10(8):1612-1617 2012
Fayna Garcia-Martin, Hiroshi Hinou, Takahiko Matsushita, Syun Hayakawa, Shin-Ichiro Nishimura

An Efficient Approach for the Characterization of Mucin-Type Glycopeptides: The Effect of O-Glycosylation on the Conformation of Synthetic Mucin Peptides
,Chemistry - A European Journal,17(8):2393-2404 2011
Ryo Hashimoto, Naoki Fujitani, Yasuhiro Takegawa, Masaki Kurogochi, Takahiko Matsushita, Kentaro Naruchi, Naoki Ohyabu, Hiroshi Hinou, Xiao Dong Gao, Naomi Manri, Hiroyuki Satake, Akihito Kaneko, Takeshi Sakamoto, Shin-Ichiro Nishimura

Chemical Synthesis, Folding, and Structural Insights into O-Fucosylated Epidermal Growth Factor-like Repeat 12 of Mouse Notch-1 Receptor
,Journal of the American Chemical Society,132(42):14857-14865 2010
Kazumi Hiruma-Shimizu, Kensaku Hosoguchi, Yan Liu, Naoki Fujitani, Takashi Ohta, Hiroshi Hinou, Takahiko Matsushita, Hiroki Shimizu, Ten Feizi, Shin-Ichiro Nishimura

Sialic Acid-focused Quantitative Mouse Serum Glycoproteomics by Multiple Reaction Monitoring Assay
,Molecular & Cellular Proteomics,9(11):2354-2368 2010
Masaki Kurogochi, Takahiko Matsushita, Maho Amano, Jun-ichi Furukawa, Yasuro Shinohara, Masato Aoshima, Shin-Ichiro Nishimura

Artificial Golgi Apparatus: Globular Protein-like Dendrimer Facilitates Fully Automated Enzymatic Glycan Synthesis
,Journal of the American Chemical Society,132(46):16651-16656 2010
Takahiko Matsushita, Izuru Nagashima, Masataka Fumoto, Takashi Ohta, Kuriko Yamada, Hiroki Shimizu, Hiroshi Hinou, Kentaro Naruchi, Takaomi Ito, Hirosato Kondo, Shin-Ichiro Nishimura

Unexpected Tolerance of Glycosylation by UDP-GalNAc:Polypeptide -N-Acetylgalactosaminyltransferase Revealed by Electron Capture Dissociation Mass Spectrometry: Carbohydrate as Potential Protective Groups
,Biochemistry,49(28):5929-5941 2010
Yayoi Yoshimura, Takahiko Matsushita, Naoki Fujitani, Yasuhiro Takegawa, Haruhiko Fujihara, Kentarou Naruchi, Xiao-Dong Gao, Naomi Manri, Takeshi Sakamoto, Kentaro Kato, Hiroshi Hinou, Shin-Ichiro Nishimura

Functional Neoglycopeptides: Synthesis and Characterization of New Class MUC1 Glycoprotein Models Having Core 2-based O-Glycan and Complex-type N-Glycan Chains
,Biochemistry,48(46):11117-11133 2009
Takahiko Matsushita, Reiko Sadamoto, Naoki Ohyabu, Hideki Nakata, Masataka Fumoto, Naoki Fujitani, Yasuhiro Takegawa, Takeshi Sakamoto, Masaki Kurogochi, Hiroshi Hinou, Hiroki Shimizu, Takaomi Ito, Kentaro Naruchi, Hiroko Togame, Hiroshi Takemoto, Hirosato Kondo, Shin-Ichiro Nishimura

An Essential Epitope of Anti MUC1 Monoclonal Antibody KL-6 Revealed by Focused Glycopeptide Library
,Journal of the American Chemical Society,131(47):17102-17109 2009
Naoki Ohyabu, Hiroshi Hinou, Takahiko Matsushita, Ryukou Izumi, Hiroki Shimizu, Keiko Kawamoto, Yoshito Numata, Hiroko Togame, Hiroshi Takemoto, Hirosato Kondo, Shin-Ichiro Nishimura

Chemical and Enzymatic Synthesis of Neoglycolipids in the Presence of Cyclodextrins
,Tetrahedron Letters,49(21):3413-3418 2008
Izuru Nagashima, Hiroki Shimizu, Takahiko Matsushita, Shin-Ichiro Nishimura

Construction of Highly Glycosylated Mucin-Type Glycopeptides Based on Microwave-Assisted Solid-Phase Syntheses and Enzymatic Modifications
,The Journal of Organic Chemistry,71(8):3051-3063 2006
Takahiko Matsushita, Hiroshi Hinou, Masataka Fumoto, Masaki Kurogochi, Naoki Fujitani, Hiroki Shimizu, Shin-Ichiro Nishimura

Construction and Structural Characterization of Versatile Lactosaminoglycan-related Compound Library for the Synthesis of Complex Glycopeptides and Sphingoglycolipids
,The Journal of Organic Chemistry,71(26):9609-9621 2006
Kentarou Naruchi, Tomoki Hamamoto, Masaki Kurogochi, Hiroshi Hinou, Hiroki

High-throughput protein glycomics: Combined Use of Chemoselective Glycoblotting and MALDI-TOF/TOF Mass Spectrometry
,Angewandte Chemie International Edition,44(1):91-96 2005
Shin-Ichiro Nishimura, Kenichi Niikura, Masaki Kurogochi, Takahiko Matsushita, Masataka Fumoto, Hiroshi Hinou, Ryousuke Kamitani, Hiroaki Nakagawa, Kisaburo Deguchi, Nobuaki Miura, Kenji Monde, Hirosato Kondo

Rapid Microwave-Assisted Solid-Phase Glycopeptide Synthesis
,Organic Letters,7(5):877-880 2005
Takahiko Matsushita, Hiroshi Hinou, Masaki Kurogochi, Hiroki Shimizu, Shin-Ichiro Nishimura

Molecular Transporter Between Polymer Platforms: Highly Efficient Chemoenzymatic Glycopeptide Synthesis by the Combined Use of Solid-Phase andWater-Soluble Polymer Supports
,Angewandte Chemie International Edition,44(17):2534-2537 2005
Masataka Fumoto, Hiroshi Hinou, Takahiko Matsushita, Masaki Kurogochi, Takashi Ohta, Takaomi Ito, Kuriko Yamada, Akio Takimoto, Hirosato Kondo, Toshiyuki Inazu, Shin-Ichiro Nishimura

Post-Translational Modification of Proteins: Facile and Efficient Procedure for the Identificationof O-Glycosylation Sites by MALDI-LIFT-TOF/TOF Mass Spectrometry
,Angewandte Chemie International Edition,43(31):4071-4075 2004
Masaki Kurogochi, Takahiko Matsushita, Shin-Ichiro Nishimura