Diurnal and Thermal Variations in an Oxidative Stress Biomarker of Pseudanabaena foetida: A Model for Environmental Adaptation in Aquatic EcosystemsMizanur Rahman; Takashi Asaeda; Kiyotaka Fukahori; Md Harun Rashid; Akihiko Matsuo
Stresses,
Volume：5,
Number：1,
First page：20,
Last page：20, Mar. 2025,
[Reviewed]Pseudanabaena foetida, a filamentous cyanobacterium, is highly sensitive to temperature and light intensity. This study explores its oxidative stress responses under diurnal light intensities in temperature variations, utilizing hydrogen peroxide (H2O2) normalized to optical density (OD730) levels (H2O2/OD730) as a biomarker. The adequate P. foetida cells were distributed to three incubators for 22 days at 30 °C, 20 °C, and 10 °C. Light intensity varied over the course of the day, increasing from morning to a maximum at noon, followed by a gradual decrease until evening. Measurements of H2O2, protein, chlorophyll a (Chl a), and catalase (CAT) activity were carried out every three hours, starting at 6:00 and ending at 21:00. Protein concentration and Chl a increased between 12:00 and 15:00 for every temperature. H2O2, CAT, and H2O2/OD730 increased until 15:00 and decreased afterwards for each temperature. The CAT was directly proportional to P. foetida’s H2O2 concentration or cell biomass (H2O2/OD730), which indicates oxidative stress responses and a defense mechanism. The reduced temperature (30 °C to 20 °C and 30 °C to 10 °C) was significantly impactful on H2O2 concentration, protein concentration, and Chl a content. The model, based on P. foetida biomass, provides valuable insights into oxidative stress responses under different temperatures, with implications for understanding climate change.
MDPI AG, Scientific journal
DOI：https://doi.org/10.3390/stresses5010020DOI ID：10.3390/stresses5010020,
eISSN：2673-7140 The long-term effect of light intensities in different temperatures can play a crucial role in suppressing Microcysties aeruginosa
Md Mizanur; Rahman Masud; Takashi Asaeda; Helayaye Damitha; Lakmali Abeynayaka; Kiyotaka Fukahori
Conference: 6 th International Hybrid Conference Water resources and wetlands, First page：17, Last page：31, Sep. 2023, [Reviewed]
International conference proceedings
Citizen’s Perception on Eco-friendly Lifestyle for Conserving Endangered Oriental White Stork and Crested Ibis Bird Species in Japan – Case Studies in Toyoka, Sado and Konosu CitiesHideo Kawashima; Kiyotaka Fukahori; Mahfuza Parveen; Garu Muni Wathsala Lakpriya Gunawardena; Takashi Asaeda
International Journal of Built Environment and Sustainability,
Volume：10,
Number：3,
First page：67,
Last page：79, Aug. 2023,
[Reviewed],
[Last, Corresponding]This study aims to find out the relationship between an eco-friendly lifestyle and the attitude towards conservation of endangered Oriental White Stork and Japanese Crested Ibis bird species in Toyooka, Sado, and Konosu cities in Japan. Citizens were surveyed to assess their willingness to spend an eco-friendly lifestyle to protect the above-mentioned endangered bird species through questionnaire surveys. Ecological Mind Evaluation Scale (EM Evaluation scale) developed by Tanaka and Joh (2012) based on Hirose model and Bandura’s research on Self Efficacy was applied in this research. This Ecological Mind Evaluation Scale includes 10 factors and it was applied to develop the questionnaire survey. The factor analysis was undertaken based on 4 groups of questionnaire results; environmental awareness, attitude towards environmental conservation, environmental consciousness and self-efficacy. Factor analysis was performed using the varimax method and sample data set comprised 686 valid responses from the three study sites. The KMO values were over 0.80 indicating the sampling was adequate. According to the factor analysis Sado city scored high for environmental awareness and environment consciousness. Toyooka city scored high on self-efficacy and environmental consciousness. Konosu city was compared with the Toyooka and Sado cities, where more advanced habitat conservation activities are already implemented. Findings displayed that the citizens in Konosu city had low awareness about these bird conservation activities, however, they are willing to contribute in such activities in the future. Thus, this study demonstrated that by engaging in environmentally friendly lifestyles, the citizens can contribute to habitat conservation of endangered bird species
Penerbit UTM Press, Scientific journal
DOI：https://doi.org/10.11113/ijbes.v10.n3.1119DOI ID：10.11113/ijbes.v10.n3.1119,
ISSN：1511-1369,
eISSN：2289-8948 An Assessment of the Effects of Light Intensities and Temperature Changes on Cyanobacteria’s Oxidative Stress via the Use of Hydrogen Peroxide as an IndicatorMizanur Rahman; Takashi Asaeda; Helayaye Damitha Lakmali Abeynayaka; Kiyotaka Fukahori
Water,
Volume：15,
Number：13,
First page：2429,
Last page：2429, Jun. 2023,
[Reviewed]Humans and other organisms are adversely affected by cyanobacterial blooming. This study aims to investigate the long-term effects of light intensities and different temperatures on Phormidium ambiguum and Pseudanabaena foetida. Enough P. ambiguum and P. foetida cells were acclimated for 24 days at 30 °C, 20 °C, and 10 °C in separate incubators. The starting day sample was collected after 24 days of acclimatization, and a second sample was collected seven days later at light intensities of 10, 30, 50, 200, and 600 µmol m−2 s−1 for each temperature. The optical density (OD730), hydrogen peroxide (H2O2) concentration, protein content, chlorophyll-a (Chl-a) concentration, and catalase (CAT) activity were measured. Light intensity changes soon after collection resulted in nearly identical starting day samples at each individual temperature. The H2O2 concentration and algal biomass increased until a light intensity of 200 µmol m−2 s−1 was reached and decreased afterward in each temperature for both species after seven days. In association with an increasing H2O2 concentration, the Chl-a concentration decreased after 50 µmol m−2s−1 of light intensity, affecting the protein content. The algal biomass was significantly lower at 10 °C compared to 30 °C. The CAT activity increased proportionately with the H2O2 concentration and algal biomass. Therefore, water bodies in the field can be illuminated with long-term high light intensities in different temperatures to reduce algal biomass.
MDPI AG, Scientific journal
DOI：https://doi.org/10.3390/w15132429DOI ID：10.3390/w15132429,
eISSN：2073-4441 Hydrogen Peroxide Measurement Can Be Used to Monitor Plant Oxidative Stress Rapidly Using Modified Ferrous Oxidation Xylenol Orange and Titanium Sulfate Assay CorrelationMizanur Rahman; Takashi Asaeda; Kiyotaka Fukahori; Fumiko Imamura; Akio Nohara; Masaomi Matsubayashi
International Journal of Plant Biology,
Volume：14,
Number：3,
First page：546,
Last page：557, Jun. 2023,
[Reviewed]Various methodologies, sensitivities, and types of interference affect the quantification of plant hydrogen peroxide (H2O2) concentration. Modified ferrous oxidation xylenol orange (eFOX) assay and titanium sulfate (Ti(SO4)2 assay are relatively accessible methods. However, their correlation is unknown, for example whether we can get the same results for different species in different environments. Leaf samples of Ambrosia trifida, Solidago altissima, Artemisia princeps, and Sicyos angulatus were collected from a riparian vegetation zone on sunny days. The H2O2 concentration in the plant leaves was evaluated in two groups. Nonfrozen leaf samples were prepared for analysis soon after arriving at the laboratory, and frozen leaf samples were stored at −80 °C for 25 days and prepared afterwards. The eFOX assay can measure even lower fluctuations in H2O2 concentration than the Ti(SO4)2 assay. A substantial correlation was observed between nonfrozen and frozen samples in the eFOX (r = 0.879, p < 0.001) and Ti(SO4)2 assays (r = 0.837, p < 0.001). Sample weight did not affect H2O2 quantification. Each species showed a substantial correlation between the eFOX and Ti(SO4)2 assays in nonfrozen conditions (Ambrosia trifida (r = 0.767, p < 0.001), Solidago altissima (r = 0.583, p < 0.001), Artemisia princeps (r = 0.672, p < 0.001), and Sicyos angulatus (r = 0.828, p < 0.001)). Therefore, both methods can be utilized easily and rapidly to quantify oxidative stress using H2O2.
MDPI AG, Scientific journal
DOI：https://doi.org/10.3390/ijpb14030043DOI ID：10.3390/ijpb14030043,
eISSN：2037-0164 駅前広場における繋がりを考慮した屋根の整備水準の評価 清水 祥貴; 深堀 清隆
Volume：9,
Number：3,
First page：11,
Last page：23, Apr. 2023,
[Reviewed],
[Last, Corresponding]DOI：https://doi.org/10.14954/jste.9.3_11DOI ID：10.14954/jste.9.3_11 Tokyo Street Project in Ulaanbaatar and Residents' Mindset of Community Development
Fukahori K.; Amarjargal N.; Ebe A.; Gonchigbat I.
International Conference on Construction and Innovation 2019, First page：76, Last page：82, 2019, [Invited], [Lead]
International conference proceedings
Evaluation of the influence of roadside non-walking spaces on the pedestrian environment of a Japanese urban street Yi Kang; Kiyotaka Fukahori; Yoichi Kubota
Sustainable Cities and Society,
Volume：43,
First page：21,
Last page：31, Nov. 2018,
[Reviewed],
[Last, Corresponding]Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/j.scs.2018.07.009DOI ID：10.1016/j.scs.2018.07.009,
ISSN：2210-6707 A STUDY ON SPATIAL LEGIBILITY OF STREET NETWORK AROUND RAILWAY STATIONYi KANG; Kiyotaka FUKAHORI; Yoichi KUBOTA; G. M. W. L. GUNAWARDENA
Journal of JSCE,
Volume：5,
Number：1,
First page：87,
Last page：100, 2017,
[Reviewed],
[Last, Corresponding]Japan Society of Civil Engineers, Scientific journal
DOI：https://doi.org/10.2208/journalofjsce.5.1_87DOI ID：10.2208/journalofjsce.5.1_87,
eISSN：2187-5103 Visual Complexity Analysis Using Taxonomic Diagrams of Figures and Backgrounds in Japanese Residential StreetscapesG. M. W. L. Gunawardena; Yoichi Kubota; Kiyotaka Fukahori
Urban Studies Research,
Volume：2015,
First page：1,
Last page：12, Sep. 2015,
[Reviewed],
[Last, Corresponding]This study was conducted with the objective of finding the perceivable effect of figures and backgrounds on residential streetscapes and their connections in visual complexity. The visual complexity depends on the extent of information a viewer can observe from a visible area. The information includes a number of visual elements along the streetscapes and their diversity and interconnections. The aim of this research was to analyze the structural hierarchical visual complexity of the streetscapes, caused by varied spatial arrangement and numerous spatial connections of the perceivable visual elements. The visual elements of 60 residential streetscapes were classified into figures and backgrounds using human perception of 20 subjects. The identified figures and backgrounds were arranged in a taxonomic diagram representing their connections. These taxonomic diagrams reflect the structural hierarchical visual complexity. Finally, taxonomic entropy was applied to statistically analyze the structural hierarchical visual complexity. When the taxonomic diagram is vertically and horizontally lengthy and the arrangement of the elements of taxonomic diagram is irregular, the complexity increases depicting a large number of figures whose spatial connections impart a high visual complexity to the streetscapes.
Hindawi Limited, Scientific journal
DOI：https://doi.org/10.1155/2015/173862DOI ID：10.1155/2015/173862,
ISSN：2090-4185,
eISSN：2090-4193 A Study on Visual Diversity of Japanese Residential Streetscapes Garu Muni Wathsala Lakpriya Gunawardena; Kubota Yoichi; Kiyotaka Fukahori
Journal of Civil Engineering and Architecture,
Volume：9,
Number：5, May 2015,
[Reviewed],
[Last, Corresponding]David Publishing Company, Scientific journal
DOI：https://doi.org/10.17265/1934-7359/2015.05.015DOI ID：10.17265/1934-7359/2015.05.015,
ISSN：1934-7359,
eISSN：1934-7367 The Influence of a Riverside Landscape on Imaginary Behavior
Kiyotaka Fukahori; Ayako Sato; Yoichi Kubota
Journal of Habitat Engineering and Design, Volume：5, Number：1, First page：1, Last page：14, Mar. 2013, [Reviewed], [Lead]
English, Scientific journal
Effect of Visual Stimuli of Planned Residential Streetscapes on Way Finding Behavior of First Time Visitors
G.M.W.L.Gunawardena; Kubota Y.; Fukahori K.
FARU International Research Symposium 2013 at Hambantota,Sri Lanka, 2013
Symposium
Walkability Evaluation of Streetscapes: Development of Prediction Equations for Walking Needs of Tourists
Ganga N.Samarasekara; Fukahori K.; Kubota Y.
Proceedings of International Symposium on Advances in Civil and Environmental Engineering Practices for Sustainable Development, First page：110, Last page：117, 2012, [Reviewed], [Last, Corresponding]
International conference proceedings
Environmental Correlates That Provide Walkability Cues for Tourists: An Analysis Based on Walking Decision NarrationsGanga N. Samarasekara; Kiyotaka Fukahori; Yoichi Kubota
Environment and Behavior,
Volume：43,
Number：4,
First page：501,
Last page：524, Jan. 2011,
[Reviewed],
[Last, Corresponding]Tourists and others who are unfamiliar with an environment may be sensitive to environmental cues when choosing their walking routes. In this study, we combined inductive and deductive approaches to evaluate walkability cues. We defined a set of walkability variables by analyzing the narratives of participants, who walked along one of 19 diverse routes. These cues were then supplemented with environmental and walkability variables from Mehrabian and Russel scales (Russel et al., 1981) and SPACES (Pikora, 2003), resulting in a total of 48 descriptors. Using the 48 descriptors, 60 diverse photos of streetscape views were rated, and we identified 14 relevant walkability correlates. Using principal component analysis, we identified six components that best predicted walking decisions: safety from traffic, comfort of walking area, environmental appearance, activity potential, shade and exploration. These results suggest that real walkers make more finely grained walking judgments than those measured by current, conceptualized walkability scales.
SAGE Publications, Scientific journal
DOI：https://doi.org/10.1177/0013916510379350DOI ID：10.1177/0013916510379350,
ISSN：0013-9165,
eISSN：1552-390X 景観評価における没入型VR 装置CAVE の導入可能性と有効性の検証
小島 翼; 早川雄喜; 深堀清隆; 窪田陽一
Volume：9, First page：25, Last page：36, Dec. 2010, [Reviewed]
Effect of street trees on spatial cognition in residential areas: an investigation based on development perspective
Ganga N.Samarasekara; Fukahori K.; Yoichi Kubota
Volume：7, First page：75, Last page：86, Dec. 2009, [Reviewed]
橋梁の夜景照明の視覚的効果に関する研究
高橋彩人; 深堀清隆; 窪田陽一
Volume：7, First page：97, Last page：108, Dec. 2009, [Reviewed]
街路空間構成からみたランドマークポテンシャルの評価
今井浩司; 深堀清隆; 窪田陽一
Volume：4, First page：67, Last page：74, Dec. 2008, [Reviewed]
植栽形態と仮想的テリトリーを考慮した街路の空間密度評価
小木学; 深堀清隆; 窪田陽一
Volume：5, First page：97, Last page：108, Dec. 2008, [Reviewed]
Cognition Affected by Urban Vegetation:Investigation at Perspective and Route Knowledge Level
Ganga N. Samarasekara; Fukahori K.; Kubota
Volume：2, First page：53, Last page：64, Dec. 2007, [Reviewed]
歩道におけるセンサー照明の活用形態と光環境
山口剛志; 窪田陽一; 深堀清隆
Volume：2, First page：23, Last page：34, 2007, [Reviewed]
情報認知量に着目した屋外広告物の視覚特性分析
長岡宏樹,窪田陽一,深堀清隆
Volume：2, First page：65, Last page：72, 2007, [Reviewed]
電線・電柱による錯綜感に関する研究
小山暁; 窪田陽一; 深堀清隆; 椎貝英仁
Volume：3, First page：95, Last page：102, 2007, [Reviewed]
埼玉県に現存する煉瓦水門の景観特性と保全のあり方に関する研究
宍戸勇気,深堀清隆,窪田陽一,三ツ畑紀子
Volume：26, First page：59, Last page：71, 2007, [Reviewed], [Last]
都市河川に架かる歴史的橋梁の構造形態を考慮した夜景照明手法
狩野哲志; 窪田陽一; 深堀清隆
Volume：1, First page：173, Last page：184, 2006, [Reviewed], [Last]
Cognition of Road Direction Affected by Geometric Features of Perspective View
Yagi H.; Fukahori K.; Kubota Y.
Proceedings of the 6th International Symposium for Environment-behavior Studies, First page：220, Last page：227, 2004
International conference proceedings
Evaluation of Lighting Environment of Street with Sensor-controlled Lighting
Fukahori K.; Kubota Y.; Matsumura M.; Takahashi O.
Proceedings of the 6th International Symposium for Environment-behavior Studies, First page：259, Last page：266, 2004, [Lead]
International conference proceedings
The role of design elements on the cost-effectiveness of streetscape improvement Kiyotaka Fukahori; Yoichi Kubota
Landscape and Urban Planning,
Volume：63,
Number：2,
First page：75,
Last page：91, Apr. 2003,
[Reviewed],
[Lead, Corresponding]Elsevier BV, Scientific journal
DOI：https://doi.org/10.1016/s0169-2046(02)00180-9DOI ID：10.1016/s0169-2046(02)00180-9,
ISSN：0169-2046 Decision support system for selecting design method in urban street improvement
Fukahori K.; Kubota Y.
Proceedings of the 7th International Conference on Computers in Urban Planning & Urban Management, 2001, [Reviewed], [Lead]
International conference proceedings
Consistency Evaluation of Landscape Design by a Decision Support SystemKiyotaka Fukahori; Yoichi Kubota
Computer-Aided Civil and Infrastructure Engineering,
Volume：15,
Number：5,
First page：342,
Last page：354, Sep. 2000,
[Reviewed],
[Lead]The planning of road structures must take into consideration both structural design and aesthetic design. Road planners and designers must keep in mind aesthetic design in the planning stages. In Japan, various design techniques have been established, and many design guidelines and design manuals have been compiled. However, most design techniques are derived from empirical knowledge, and their explanations are often subjective. We have developed a decision support system in landscape planning to simplify complicated processes and avoid fuzziness in decision making. We have collected a large amount of empirical knowledge and have created a database for use in developing design techniques. The main system supports designers and allows planners to select suitable design techniques considering environmental conditions and design policy based on a knowledge‐based system. The introduction of three concepts—visual importance, visual suitability, and four kinds of design consistency—allows solution of certain design problems.
Wiley, Scientific journal
DOI：https://doi.org/10.1111/0885-9507.00198DOI ID：10.1111/0885-9507.00198,
ISSN：1093-9687,
eISSN：1467-8667 Study on the Visual Impact of High-rise Buildings as Landmarks in the Urban Setting
Wen Yue Ho; Kubota Y.; Fukahori K.
Proceedings of International Symposium on City Planning, First page：113, Last page：121, 2000, [Reviewed]
International conference proceedings
Quantification of Visual Dominance for Landscape Evaluation of Road Structures
Fukahori K.; Kubota Y.
Proceedings of the 6th International Conference on Computers in Urban Planning & Urban Management, 1999, [Reviewed], [Lead]
景観設計支援システム利用におけるデザイン技法間の内的整合性評価
深堀 清隆; 窪田 陽一
Volume：8, First page：143, Last page：150, 1999, [Reviewed], [Lead]
Decision Support by Knowledge Based System in Landscape Engineering
Fukahori K.; Kubota Y.
Proceedings of The First International Conference on New Information Technologies for Decision Making in Civil Engineering, Volume：1, First page：577, Last page：586, 1998, [Reviewed], [Lead]
English, International conference proceedings
Effects of the Structure of Open Space on the Visual Accessibility of Railway Stations in Urbanized Area
Elhady G.; Kubota Y.; Fukahori K.
Proceedings of International Symposium on City Planning, First page：421, Last page：430, 1998, [Reviewed]
English, International conference proceedings
Correlation between Build-up Density and Visual Evaluation of residential Street
Fukahori K.; Kubota Y.; Bukasa A.
Proceedings of International Symposium on City Planning, First page：367, Last page：373, 1997, [Reviewed], [Lead]
Development of Visual Assessment System for Planning and Design of Expressway
Fukahori K.; Kubota Y.; Otomo M.; Yagi H.,Masaki
Proceedings of The Seventh International Conference on Computing in Civil and Building Engineering, Volume：1, First page：715, Last page：720, 1997, [Reviewed], [Lead]
International conference proceedings
法面保護工選定知識ベースを有する景観設計技法検索システム
深堀 清隆; 窪田 陽一; 大友 正晴; 八木 英夫; 高瀬 一希
Volume：6, First page：25, Last page：32, 1997, [Reviewed], [Lead]
高速道路景観における跨道橋の主対象としての認識に関する基礎的研究
深堀清隆; 窪田陽一; Nguyen Ba Hoang; 政木英一
Volume：42, First page：527, Last page：534, Mar. 1996, [Reviewed], [Lead]
動的景観シミュレーションシステムを用いた跨道橋梁群の景観評価手法
深堀清隆; 窪田陽一
Volume：39A, First page：583, Last page：594, 1993, [Reviewed], [Lead]
