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塚原　伸治 ツカハラ シンジ

■所属部署名 理工学研究科　生命科学部門 ■電話番号
■職名 教授 ■FAX番号
■住所 埼玉県さいたま市桜区下大久保255 ■メールアドレス stsuka＠mail.saitama-u.ac.jp
■ホームページURL

プロフィール

兼担研究科・学部

理学部 生体制御学科

研究分野

神経内分泌学

現在の研究課題

脳の性分化機構の解明
キーワード：性ステロイドホルモン , 性的二型核 , 性差

所属学会

所属学会
日本神経科学会
日本神経内分泌学会
日本比較内分泌学会
日本繁殖生物学会
日本動物学会
北米神経科学会

学歴

出身大学院・研究科等
1999 , 名古屋大学大学院 , 博士後期 , 生命農学研究科 , 修了
1996 , 名古屋大学大学院 , 博士前期 , 生命農学研究科 , 修了
出身学校・専攻等(大学院を除く)
1994 , 名古屋大学 , 農学部 , 卒業
取得学位
博士（農学） , 名古屋大学

受賞学術賞

2010 , 室内環境学会ポスター賞
2008 , 室内環境学会ポスター賞
2008 , 日本神経内分泌学会川上賞
2001 , 日本性機能学会学術総会会長賞

研究職歴等

研究職歴
2005 - 2009 , 独立行政法人国立環境研究所　主任研究員
2002 - 2004 , 神戸大学大学院自然科学研究科　助手
1999 - 2002 , 早稲田大学人間総合研究センター　助手
1996 - 1999 , 日本学術振興会　特別研究員DC1
研究職歴以外の職歴
2011 , 東京大学　非常勤講師
2009 , 独立行政法人国立環境研究所　客員研究員
2006 - 2009 , 千葉大学大学院薬学研究院　客員准教授

研究活動業績

研究業績(著書・発表論文等)

著書
脳とホルモンの行動学　わかりやすい行動神経内分泌学　第2版
西村書店:41-58 202303
ISBN:784867060438
塚原伸治、他３１名

下位脳幹の性差
裳華房,脳の性分化:84-92 2006
塚原伸治

神経核形成のメカニズム（アポトーシス）
裳華房,脳の性分化:107-121 2006
塚原伸治、掛山正心

みんなの生命科学
化学同人:78-86, 104-111, 147-159, 170-171
北口哲也、塚原伸治、坪井貴司、前川文彦

ホルモンから見た生命現象と進化シリーズIV：求愛・性行動と脳の性分化
裳華房:70-93
浜崎浩子、小林牧人、棟方有宗、小澤一史、豊田ふみよ、塚原伸治、吉国道庸

論文
Ethynylestradiol feminizes gene expression partly in testis developing as ovotestis and disrupts asymmetric Müllerian duct development by eliminating asymmetric gene expression in Japanese quail embryos.
Toxicological Sciences,accepted 2024
Abe N, Sakiyama A, Suzuki M, Win-Shwe TT, Suzuki T, Kawashima T, Tsukahara S

Two step actions of testicular androgens in the organization of a male-specific neural pathway from the medial preoptic area to the ventral tegmental area for modulating sexually motivated behavior.
Journal of Neuroscience,43:7322-7336 2023
Morishita M, Kobayashi K, Mitsuzuka M, Takagi R, Ono K, Momma R, Tsuneoka Y, Horio S, Tsukahara S

In ovo o,p'-DDT exposure induces malformation of reproductive organs and alters the expression of genes controlling sexual differentiation in Japanese quail embryo.
Journal of Applyed Toxicology,in press:DOI: 10.1002/jat.4571 2023
Win-Shwe TT, Abe N, Sakiyama A, Suzuki M, Sano K, Kawashima T, Tsukahara S

Perinatal exposure to diesel exhaust origin secondary organic aerosol induces autism-like behavior in rats.
International Journal of Molecular Sciences,22:doi: 10.3390/ijms22020538 2021
Win-Shwe TT, Tha-Thu CK, Fujitani Y, Tsukahara S, Hirano S

The role of central corticotrophin-releasing factor receptor signalling in plasma glucose maintenance through ghrelin secretion in calorie-restricted mice.
Journal of Neuroendocrinology,33:doi: 10.1111/jne.12961 2021
Kimura R, Kondo D, Takemi S, Fujishiro M, Tsukahara S, Sakai T, Sakata I

Neuronal activation of the sexually dimorphic nucleus of the preoptic area in female and male rats during copulation and its sex differences.
Neuroscience Letters,755:doi: 10.1016/j.neulet.2021.135915 2021
Morishita M, Kamada A, Tsukahara S

Sexually dimorphic expression of sexual differentiation genes in the internal genital organs of Japanese quail embryos.
General and Comparative Endocrinology,314:doi: 10.1016/j.ygcen.2021.113917 2021
Tsukahara S, Morishita M, Sasaki S, Wakayama K, Kobayashi K, Ohno K, Kawashima T

Estrogenic action by tris(2,6-dimethylphenyl) phosphate impairs the development of female reproductive functions.
Environment International,138:doi: 10.1016/j.envint.2020.105662 2020
Sano K, Matsukami H, Suzuki G, Htike NTT, Morishita M, Win-Shwe TT, Hashimoto S, Kawashima T, Isobe T, Nakayama SF, Tsukahara S, Maekawa F

Actions of peripubertal gonadal steroids in the formation of sexually dimorphic brain regions in mice.
Endocrinology,161, 6:1-17 2020
Morishita M, Koiso R, Tsukahara S

Detection and characterization of estrogen receptor beta expression in the brain of newly developed transgenic mice.
Neuroscicence,438:182-197 2020
Sagoshi S, Maejima S, Morishita M, Takenawa S, Hasunuma K, Otubo A, Takanami K, Takahashi A, Sakamoto T, Sakamoto H, Tsukahara S, Ogawa S

The glutamatergic system in the preoptic area is involved in the retention of maternal behavior in maternally experienced female rats.
Psychoneuroendocrinology,120:doi: 10.1016/j.psyneuen.2020.104792, 2020 2020
Okino E, Morita S, Hoshikawa Y, Tsukahara S

Estrogenic regulation of social behavior and sexually dimorphic brain formation.
Neuroscience and Biobehavioral Reviews,110:46-59 2020
Ogawa S, Tsukahara S, Choleris E, Vasudevan N

Sexually dimorphic formation of the preoptic area and the bed nucleus of the stria terminalis by neuroestrogens.
Frontiers in Neuroscience,14:doi:10.3389/fnins.2020.00797, 2020 2020
Tsukahara S, Morishita M

Neonatal septal lesions prevent behavioral defeminization caused by neonatal treatment with estradiol in female rats.
Neuroscience Letters,694:80-85 2019
Kanaya M, Tsukahara S, Yamanouchi K

Female-biased sexual dimorphism of corticotropin-releasing factor neurons in the bed nucleus of the stria terminalis.
Biology of Sex Differences,10:doi: 10.1186/s13293-019-0221-2 2019
Uchida K, Otsuka H, Morishita M, Tsukahara S, Sato T, Sakimura K, Itoi K

Region-specific effects of copulation on dendritic spine morphology and gene expression related to spinogenesis in the medial preoptic nucleus of male rats.
Psychoneuroendocrinology,108:1-13 2019
Nakashima S, Morisita M, Ueno K, Tsukahara S

Strain differences in intermale aggression and possible factors regulating increased aggression in Japanese quail.
General and Comparative Endocrinology,256:63-70 2018
Maekawa F, Nagino K, Yang J, Htike NTT, Tsukahara S, Ubuka T, Tsutsui K, Kawashima T

Neuronal activity in the sagittalis nucleus of the hypothalamus after ovarian steroid hormone manipulation and sexual behavior in female rat.
Neuroscience Letters,671:25-28 2018
Balabanov IE, Matsuda KI, Mori H, Yamada S, Kitagawa K, Yamamoto Y, Tsukahara S, Tanaka M

Temporal expression patterns of genes related to sex steroid action in sexually dimorphic nuclei during puberty.
Frontiers in Endocrinology,9:doi: 10.3389/fendo.2018.00213 2018
Kanaya M, Morishita M, Tsukahara S

Sexual experience reduces neuronal activity in the central part of the medial preoptic nucleus in male rats during sexual behavior.
Neuroscience Letters,685:155-159 2018
Yamaguchi S, Abe Y, Maejima S, Tsukahara S

VGF in the medial preoptic nucleus increases sexual activity following sexual arousal induction in male rats.
Endocrinology,159(12):3993-4005 2018
Maejima S, Abe Y, Yamaguchi S, Musatov S, Ogawa S, Kondo Y, Tsukahara S

Moxd1 is a marker for sexual dimorphism in the medial preoptic area, bed nucleus of the stria terminalis and medial amygdala.
Frontiers in Neuroanatomy,11, 26:doi: 10.3389/fnana.2017.00026 2017
Tsuneoka Y, Tsukahara S, Yoshida S, Takase K, Oda S, Kuroda M, Funato H

Gonadal hormone-dependent sexual differentiation of a female-biased sexually dimorphic cell group in the principal nucleus of the bed nucleus of the stria terminalis in mice.
Endocrinology, ,158, 10:3512-3525 2017
Morishita M, Maejima S, Tsukahara S

Sexual behavior-associated c-Fos indcuation in the sagittalis nucleus of the hypothalamus in male rat.
Neuroscience Letters,661:104-107 2017
Matsuda KI, Uchiyama K, Mori H, Maejima S, Yamaguchi S, Tanaka M, Tsukahara S

Exposure of BALB/c mice to diesel engine exhaust origin secondary organic aerosol (DE-SOA) during the developmental stages impairs the social behavior in adult life of the mice.
Frontiers in Neuroscience,9, 254: doi: 10.3389/fnins.2015.00524 2016
Tin-Tin Win-Shwe, Chaw Kyi-Tha-Thu, Yadanar Moe, Fujitani Y, Tsukahara S & Hirano S

Prenatal Exposure to Arsenic Impairs Behavioral Flexibility and Cortical Structure in Mice.
Frontiers in Neuroscience,10, 137:doi: 10.3389/fnins.2016.00137 2016
Aung KH, Kyi-Tha-Thu C, Sano K, Nakamura K, Tanoue A, Nohara K, Kakeyama M, Tohyama C, Tsukahara S & Maekawa F

Pubertal activation of estrogen receptor α in the medial amygdala is essential for the full expression of male social behavior in mice.
Proceedings of the National Academy of Sciences of the United States of America,13, 27:7632-7637 2016
Sano K, Nakata M, Musatov S, Morishita M, Sakamoto T, Tsukahara S & Ogawa S

Arsenic exposure induces unscheduled mitotic S phase entry coupled with cell death in mouse cortical astrocytes.
Frontiers in Neuroscience,10, 297:doi: 10.3389/fnins.2016.00297 2016
Htike NT, Maekawa F, Soutome H, Sano K, Maejima S, Kyaw Htet Aung, Tokuda M & Tsukahara S

A comparative study of sex difference in calbindin neurons among mice, musk shrews, and Japanese quails.
Neuroscience Letters,631:63-69 2016
Moe Y, Tanaka T, Morishita M, Ohata R, Nakahara C, Kawashima T, Maekawa F, Sakata I, Sakai T, Tsukahara S

Molecular cloning of ghrelin and characteristics of ghrelin-producing cells in the gastrointestinal tract of the common marmoset (Callithrix jacchus).
Zoological Science,33, 5:497-504 2016
Takemi S, Skata I, Tsukahara S, Yahashi S, Katsuura G, Iwashige F, Akune A, Inui A & Sakai T

A sexually dimorphic area of the dorsal hypothalamus in mice and common marmosets.
Endocrinology,157, 12:4817-4828 2016
Moe Y, Kyi-Tha-Thu C, Tanaka T, Ito H, Yahashi S, Matsuda KI, Kawata M, Katsuura G, Iwashige F, Sakata I, Akune A, Inui A, Sakai T, Ogawa S, Tsukahara S

Nano-sized secondary organic aerosol of diesel engine exhaust origin impairs olfactory-based spatial learning performance in preweaning mice.
Nanomaterials,5:1147-1162 2015
Tin-Tin Win-Shwe, Chaw Kyi-Tha-Thu, Yadanar Moe, Maekawa F, Yanagisawa R, Furuyama A, Tsukahara S, Fujitani Y & Hirano S

Sex differences in cells expressing green fluorescent protein under the control of the estrogen receptor-α promoter in the hypothalamus of mice.
Neuroscience Research,101:44-52 2015
Kyi Tha Thu C, Okoshi K, Ito H, Matsuda KI, Kawata M & Tsukahara S

A neural connection between the central part of the medial preoptic nucleus and the bed nucleus of the stria terminalis to regulate sexual behavior in male rats.
Neuroscience Letters,606:66-71 2015
Maejima S, Ohishi N, Yamaguchi S & Tsukahara S

Role of Environmental Chemical Insult in Neuronal Cell Death and Cytoskeleton Damage.
Biological and Pharmaceutical Bulletin,38:1109-1112 2015
Kyaw Htet Aung, Tsukahara S, Maekawa F, Nohara K, Nakamura K & Tanoue A

Neural, hormonal and experiential control of sex-typical expression of social behavior.
Interdisciplinary Information Sciences,21:181-187 2015
Ogawa S, Tsuda MC, Sano K, Tsukahara S & Musatov S

Involvement of hemeoxygenase-1 in di(2-ethylhexyl) phthalate (DEHP)-induced apoptosis of Neuro-2a cells.
Journal of Toxicological Sciences,39:217-229 2014
Kyaw Htet Aung, Tin-Tin Win-Shwe, Kanaya M, Takano H, Tsukahara S

Regional difference in sex steroid action on formation of morphological sex differences in the anteroventral periventricular nucleus and principal nucleus of the bed nucleus of the stria terminalis.
PLoS One,9: e112616 2014
Kanaya M, Tsuda CM, Sagoshi S, Nagata K, Morimoto C, Chaw Kyi Tha Thu, Toda K, Kato S, Ogawa S, Tsukahara S

Effects of diesel engine exhaust origin secondary organic aerosol on novel object recognition ability and maternal behavior in BALB/c mice.
International Journal of Environmental Research and Public Health,11:11286-11307 2014
Tin-Tin Win-Shwe, Fujitani Y, Chaw Kyi-Tha-Thu, Furuyama A, Tsukahara S, Nitta H, Hirano S

The mechanisms underlying sexual differentiation of behavior and physiology in mammals and birds: relative contributions of sex steroids and sex chromosomes.
Frontiers in Neuroscience,8:242 2014
Maekawa F, Tsukahara S, Kawashima T, Nohara K, Ohki-Hamazaki H

Neuroanatomy and sex differences of the lordosis-inhibiting system in the lateral septum.
Frontiers in Neuroscience,8:299 (doi: 10.3389/fnins.2014.00299) 2014
Tsukahara S, Kanaya M, Yamanouchi K

Inhibition of neurite outgrowth and alteration of cytoskeletal gene expression by sodium arsenite.
Neurotoxicology,34:226-235 2013
Kyaw Htet Aung, Kurihara R, Nakashima S, Maekawa F, Nohara K, Kobayashi T & Tsukahara S

Collapsin response mediator protein affects the number of tyrosine hydroxylase-immunoreactive neurons in the sexually dimorphic nucleus in female mice.
Developmental Neurobiology,73:502-517 2013
Iwakura T, Sakoh M, Tsutiya A, Yamashita N, Ohtani A, Tsuda M, Ogawa S, Tsukahara S, Nishihara M, Shiga Y, Goshima Y, Kato T & Ohtani-Kaneko R

Visualization and Characterization of Oestrogen Receptor a-Positive Neurons Expressing GFP under the Control of the Oestrogen Receptor a Promoter.
European Journal of Neuroscience,38:2242-2249 2013
Matsuda KI, Yanagisawa M, Sano K, Ochiai I, Musatov S, Okoshi K, Tsukahara S, Ogawa S & Kawata M

Effects of sodium arsenite on neurite outgrowth and glutamate AMPA receptor expression in mouse cortical neurons.
Neurotoxicology,37:197-206 2013
Maekawa F, Tsuboi T, Oya M, Kyaw Htet Aung, Tsukahara S, Pellerin L & Nohara K

Differential mRNA expression of neuroimmune markers in the hippocampus of infant mice following toluene exposure during brain developmental period.
Journal of Applied Toxicology,32:126-134 2012
Tin-Tin-Win-Shwe, Kunugita N, Yoshida Y, Nakajima D, Tsukahara S, Fujimaki H

Differential mRNA expression of neuroimmune markers in the hippocampus of infant mice following toluene exposure during brain developmental period.
Journal of Applied Toxicology,32:126-134 2012
Tin-Tin-Win-Shwe, Kunugita N, Yoshida Y, Nakajima D, Tsukahara S, Fujimaki H

Involvement of postnatal apoptosis on sex difference in number of cells generated during late fetal period in the sexually dimorphic nucleus of the preoptic area in rats.
Neurosci Lett.,516:290-295 2012
Kato Y, Nakashima S, Maekawa F, Tsukahara S.

Differential mRNA expression of neuroimmune markers in the hippocampus of infant mice following toluene exposure during brain developmental period.
Journal of Applied Toxicology:in press 2011
Tin-Tin-Win-Shwe, Kunugita N, Yoshida Y, Nakajima D, Tsukahara S, Fujimaki H

Effects of aromatase or estrogen receptor gene deletion on masculinization of the principal nucleus of the bed nucleus of the stria terminalis of mice.
Neuroendocrinology:in press 2011
42. Tsukahara S, Tsuda M, Kurihara R, Kato Y, Kuroda Y, Nakata M, Xiao K, Nagata K, Toda K, Ogawa S

Effects of aromatase or estrogen receptor gene deletion on masculinization of the principal nucleus of the bed nucleus of the stria terminalis of mice.
Neuroendocrinology,94:137-147 2011
Tsukahara S, Tsuda M, Kurihara R, Kato Y, Kuroda Y, Nakata M, Xiao K, Nagata K, Toda K, Ogawa S

Does early life toluene exposure alter the expression of NMDA receptor subunits and signal transduction pathway in infant mouse hippocampus?
Neurotoxicology,31(6):647-653 2010
Tin-Tin-Win-Shwe, Yoshida Y, Kunugita N, Tsukahara S, Fujimaki H

Intrauterine position affects estrogen receptor alpha expression in the ventromedial nucleus of the hypothalamus via promoter DNA methylation
Endocrinology,151(12):5775-5781 2010
Mori H, Matsuda KI, Tsukahara S, Kawata M

The effect of D-cycloserine on spatial learning performance and memory function-related gene expression in mice following toluene exposure
Journal of UOEH,32(2):127-140 2010
Win-Shwe TT, Kageyama S, Tsukahara S, Nakajima D, Fujimaki H

Up-regulation of neurotrophin-related gene expression in mouse hippocampus following low level toluene exposure
Elsevier,Neurotoxicology,31(1):85-93 2010
Win-Shwe TT, Tsukahara S, Yamamoto S, Fukushima A, KunugitaN, Arashidani K, Fujimaki H

Use of live imaging analysis for evaluation of cytotoxic chemicals that induce apoptotic cell death
Toxicology In Vitro,24(7):2012-2020 2010
Koike-Kuroda Y, Kakeyama M, Fujimaki H, Tsukahara S

脳の性分化におよぼす発達期トルエン曝露の影響と作用機序
室内環境,13(1):1-8 2010
塚原伸治、中島大介、藤巻秀和

Effects of maternal toluene exposure on testosterone levels in fetal rats
Elsevier,Toxicology Letters,185(2):79-84 2009
Tsukahara S, Nakajima D, Kuroda Y, Hojo R, Kageyama S, Fujimaki H

Measurement of toluene concentrations in the blood of fetuses of pregnant rats exposed to low concentration toluene using headspace solid phase micro extraction-gas chromatograph-mass spectrometry
日本薬学会,Journal of Health Science,55(1):50-55 2009
Nakajima D, Tsukahara S, Hojo R, Kageyama S, Goto S, Shiraishi H, Shiraishi F, Fujimaki H

Sex differences and roles of sex steroids in apoptosis of sexually dimorphic nuclei of preoptic area in postnatal rats
Blackwell,Journal of Neuroendocrinology,21(4):370-376 2009
Tsukahara S

Estrogen modulates Bcl-2 family protein expression in the sexually dimorphic nucleus of the preoptic area of postnatal rats
Neuroscience Letters,432(1):58-63 2008
Tsukahara S, Hojo R, Kuroda Y, Fujimaki H

Activation of cholecystokinin neurons in the dorsal pallium of the telencephalon is indispensable for the acquisition of chick imprinting behavior
Journal of Neurochemistry,102(5):1645-1657 2007
Maekawa F, Nakamori T, Uchimura M, Fujiwara K, Yada T, Tsukahara S, Kanamatsu T, Tanaka K, Ohki-Hamazaki H

Athymic nude mice are insensitive to low-level toluene-induced up-regulation of memory-related gene expressions in the hippocampus
Neurotoxicology,28(5):957-964 2007
Win-Shwe TT, Tsukahara S, Ahamed S, Fukushima A, Yamamoto S, Kakeyama M, Nakajima D, Goto S, Kobayashi T, Fujimaki H

Effects of low-level formaldehyde exposure on synaptic plasticity related gene expression in the hippocampus of immunized mice
Journal of Neuroimmunology,186(1-2):104-111 2007
Ahmed S, Tsukahara S, Win-Shwe TT, Yamamoto S, Kunugita N, Arashidani K, Fujimaki H

Increased hippocampal mRNA expression of neuronal synaptic plasticity related genes in mice chronically exposed to toluene at a low-level human occupational-exposure
Neurotoxicology,28(1):168-174 2007
Ahmed S, Win-Shwe TT, Yamamoto S, Tsukahara S, Kunugita N, Arashidani K, Fujimaki H

Toluene induces rapid and reversible rise of hippocampal glutamate and taurine neurotransmitter levels in mice
Toxicology Letters,168(1):75-82 2007
Win-Shwe TT, Mitsushima D, Nakajima D, Ahmed S, Yamamoto S, Tsukahara S, Kakeyama M, Goto S, Fujimaki H

Increased Fos immunoreactivity in suprachiasmatic nucleus before luteinizing hormone surge in estrogen-treated ovariectomized female rats
Neuroendocrinology,83(5-6):303-312 2006
Tsukahara S

Inhalation of low-level formaldehyde increases the Bcl-2/Bax expression ratio in the hippocampus of immunologically sensitized mice
Neuroimmunomodulation,13(2):63-68 2006
Tsukahara S, Yamamoto S, Tin-Tin-Win-Shwe, Ahmed S, Kunugita N, Arashidani K, Fujimaki H

Pituitary adenylate cyclase-activating polypeptide neurons of the ventromedial hypothalamus project to the midbrain central gray
Neuroreport,17(2):221-224 2006
Maekawa F, Fujiwara K, Tsukahara S, Yada T

Progression of the dose-related effects of estrogenic endocrine disruptors, an important factor in declining fertility, differs between the hypothalamo-pituitary axis and reproductive organs of male mice
Journal of Veterinary Medical Science,68(12):1257-1267 2006
Warita K, Sugawara T, Yue Z-P, Tsukahara S, Mutoh K, Hasegawa Y, Kitagawa H, Mori C, Hoshi N

Sex differences in the level of Bcl-2 family proteins and caspase-3 activation in the sexually dimorphic nuclei of the preoptic area in postnatal rats
Journal of Neurobiology,66(13):1411-1419 2006
Tsukahara S, Kakeyama M, Toyofuku Y

Age-related change and its sex differences in histoarchitecture of the hypothalamic suprachiasmatic nucleus of F344/N rats
Experimental Gerontology,40(3):147-155 2005
Tsukahara S, Tanaka S, Ishida K, Hoshi N, Kitagawa H

教育活動実績

授業等

調節生理学I（理学部）
調節生理学実験（理学部）
基礎生物学演習II（理学部）
調節生理学演習（理学部）
生物学実験B（理学部）
基礎生物学III（共通教育）
調節生理学輪講（理工学研究科）