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Profile
NEMOTO NAOTO
| ■Faculty: | Graduate School of Science and Engineering | ■TEL: |
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| ■Position: | Professor | ■FAX: | ||
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Profile
Assigned Class
- Faculty of Engineering
- Advanced Institute of Innovative Technology
Research
Books, Articles, etc.
- Books
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実験医学増刊「治療の可能性が広がる抗体医薬」
羊土社:97-106 202212
ISBN:978-4-7581-0407-4
土屋政幸、根本直人 -
Methods in Molecular Biology "Proteomic Profiling"
Springer-Nature:307-321 202101
ISBN:978-1-0716-1185-2
Chathuni Jayathilake and Naoto Nemoto -
Methods Mol Biol. "Genotype Phenotype Coupling"
Springer-Nature:43-56 202006
ISBN:978-1-4939-9852-4
Arai H, Kumachi S, Nemoto N. -
Evolutionary Molecular Engineering to Efficiently Direct in vitro Protein Synthesis
InTech:51-62 2012
ISBN:ISBN 978-953-51-0803-0
Manish Biyani, Madhu Biyani, Naoto Nemoto and Yuzuru Husimi - Articles
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A novel agonist for FGF receptor-1 based on homobivalent single domain antibodies binding to the receptor domain III functions as FGF2 ligand
,J Bio Chem,299(2):102804 2023
Ryo Yonehara, Shigefumi Kumachi, Kenji Kashiwagi, Kanako Wakabayashi-Nakao, Maiko Motohashi, Taihei Murakami, Teruhiko Yanagisawa, Hidenao Arai, Akikazu Murakami, Yukio Ueno, Naoto Nemoto, and Masayuki Tsuchiya -
Comprehensive analysis of transglutaminase substrate preference by cDNA display coupled with next-generation sequencing and bioinformatics
,Scientific Reports,12 :13578 2022
Jasmina Damnjanović, Nana Odake, Jicheng Fan, Maurizio Camagna, Beixi Jia, Takaaki Kojima, Naoto Nemoto, Kiyotaka Hitomi, Hideo Nakano -
生命の起源から「ペプチド」へのアプローチ
,PEPTIDE NEWSLETTER JAPAN,125 :8-11 2022
根本直人 -
cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection
,Methods in Molecular Biology,2261:307-321 2021
Chathuni Jayathilake and Naoto Nemoto -
In vitro selection of anti-gliadin single-domain antibodies from a naïve library for cDNA-display mediated immuno-PCR.
,Anal Biochem,589:doi:10.1016/j.ab.2019.113490. 2020
Jayathilake C, Kumachi S, Arai H, Motohashi M, Terai T, Murakami A, Nemoto N. -
In Vitro Construction of Large-scale DNA Libraries from Fragments Containing Random Regions using Deoxyinosine-containing Oligonucleotides and Endonuclease V.
,ACS Comb Sci.,22:165-171 2020
Yamamoto Y, Terai T, Kumachi S, Nemoto N. -
Photocrosslinking of cDNA Display Molecules with Their Target Proteins as a New Strategy for Peptide Selection.
,Molecules,25:E1472 2020
Terai T, Koike T, Nemoto N. -
NEDO Startups Future
,focus NEDO,78:18 2020
根本直人 -
高速進化技術で「抗体」デザイン・・・医薬品・診断薬への実用化へ
,埼経協ニュース(421):6 2020
根本直人 -
Selection of Peptides that Associate with Dye-Conjugated Solid Surfaces in a pH-Dependent Manner Using cDNA Display
,ACS Omega,4:7378-7384 2019
Terai, T., Anzai, H., Nemoto, N. -
A novel immuno-PCR method using cDNA display
,Anal Biochem,578:1-6 2019
Anzai H, Terai T, Jayathilake C, Suzuki T, Nemoto N. -
cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
,Bio-protocol,9: e3457 2019
Jayathilake, C., Terai, T. and Nemoto -
Selection of Peptides that Associate with Dye-Conjugated Solid Surfaces in a pH-Dependent Manner Using cDNA Display
,ACS Omega,4:7378-7384 2019
Terai, T., Anzai, H., Nemoto, N. -
A novel immuno-PCR method using cDNA display
,Analytical Biochemistry,578:1-6 2019
Anzai H, Terai T, Jayathilake C, Suzuki T, Nemoto N. -
Anti-survivin single-domain antibodies derived from an artificial library including three synthetic random regions by in vitro selection using cDNA display.
,Biochem Biophys Res Commun,503:2054-2060 2018
Suzuki T, Mochizuki Y, Kimura S, Akazawa-Ogawa Y, Hagihara Y, Nemoto N. -
Enhanced mRNA-protein fusion efficiency of a single-domain antibody by selection of mRNA display with additional random sequences in the terminal translated regions
,Biophysics and Physicobiology,14:23-28 2017
Kazuki Takahashi, Masato Sunohara, Takuya Terai, Shigefumi Kumachi and Naoto Nemoto -
In vitro Selection of Random Peptides against Artificial Lipid Bilayers: A Potential Tool to Immobilize Molecules on Membranes
,Chem Commun (Camb),53:3458-3461 2017
Shota Kobayashi, Takuya Terai, Yuki Yoshikawa, Ryoya Ohkawa, Mika Ebihara, Masahito Hayashi, Kingo Takiguchi, and Naoto Nemoto -
A chirality of biopolymer from the perspective of evolutionary molecular engineering
,Viva Origino ,44ページ:5 2017
Naoto Nemoto -
Iodoacetyl-functionalized pullulan: A supplemental enhancer for single-domain antibody-polyclonal antibody sandwich enzyme-linked immunosorbent assay for detection of survivin.
,Bioorg Med Chem Lett.,27 :4844-4848 2017
Matsushita T, Arai H, Koyama T, Hatano K, Nemoto N, Matsuoka K. -
High-throughput Selection of Pore-forming Peptides Assembling in Liposome Membranes by Combining cDNA Display Method with Cell Sorter System
,Peptide Science 2017 (Edited by Ikuo Fujii):30-31 2017
Hiroki Miyajima, Naoto Nemoto -
A Study to Select pH-dependent Phenolphthalein-binding Peptide Aptamers by cDNA Display Method
,Peptide Science 2017 (Edited by Ikuo Fujii):54-55 2017
Hiroki Anzai, Takuya Terai, and Naoto Nemoto -
Easy and rapid binding assay for functional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system
,Biology (Basel),4 (1) :161-172 2015
Tanemura Y, Mochizuki Y, Kumachi S, Nemoto N. -
Functional Analysis of Liposome Anchoring Peptide Selected by Complementary DNA display (cDNA display)
,Peptide Science (Edited by Akira Otaka):71-72 2015
Naoto Nemoto, Yuki Yoshikawa, Toshiki Miyajima, and Shota Kobayashi -
A versatile puromycin-linker using cnvK for high-throughput in vitro selection by cDNA display
,Journal of Biotechnology,212:174-180 2015
Yuki Mochizuki, Takeru Suzuki, Kenzo Fujimoto, Naoto Nemoto -
An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
,ACS Omega,1:52–57 2015
Shigefumi Kumachi, Yuzuru Husimi, and Naoto Nemoto -
Amino group binding peptide aptamers with double disulphide-bridged loops selected by in vitro selection using cDNA display.
,Chem Commun (Camb),50 (42） :5608-5610 2014
Mochizuki Y, Nishigaki K, Nemoto N. -
Versatile C-terminal specific biotinylation of proteins using both a puromycin-linker and a cell-free translation system for studying high-throughput protein-molecule interactions
,Anal Chem.,86 (17) :8535-8540 2014
Nemoto N, Fukushima T, Kumachi S, Suzuki M, Nishigaki K, Kubo T. -
Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies.
,BMC Biotechnol.,14 (1):78 2014
Sharma H, Kinoshita Y, Fujiu S, Nomura S, Sawada M, Ahmed S, Shibuya M, Shirai K, Takamatsu S, Watanabe T, Yamazaki H, Kamiyama R, Kobayashi T, Arai H, Suzuki M, Nemoto N, Ando K, Uchida H, Kitamura K, Takei O, Nishigaki K. -
An RI-nondependent high-sensitivity method for measuring the activity of glioblastoma-related O(6)-methylguanine-DNA methyltransferase (MGMT).
,Anal Biochem.,480 :82-84 2014
Hongo A, Gu R, Suzuki M, Nemoto N, Nishigaki K. -
Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method
,Biophysics,10 :55-62 2014
Harshita Sharma, Fumihito Ohtani, Parmila Kumari, Deepti Diwan, Naoko Ohara, Tetsuya Kobayashi, Miho Suzuki, Naoto Nemoto, Yoshibumi Matsushima, Koichi Nishigaki -
Detection of ultra-low levels of DNA changes by drinking water: epidemiologically important finding.
,J Biochem.,157 (2) :113-119 2014
Kumari P, Kamiseki M, Biyani M, Suzuki M, Nemoto N, Aita T, Nishigaki K. -
Systematic genome sequence differences among leaf cells within individual trees.
,BMC Genomics.,15:142 2014
Diwan D, Komazaki S, Suzuki M, Nemoto N, Aita T, Satake A, Nishigaki K. -
Optimal terminal sequences for continuous or serial isothermal amplification of dsRNA with norovirus RNA replicase
,Biophysics,10:15-23 2014
Hidenao Arai, Koichi Nishigaki, Naoto Nemoto, Miho Suzuki and Yuzuru Husimi -
An RI-nondependent high-sensitivity method for measuring the activity of glioblastoma-related O(6)-methylguanine-DNA methyltransferase (MGMT).
,Anal Biochem.,480 :82-84 2014
Hongo A, Gu R, Suzuki M, Nemoto N, Nishigaki K. -
Detection of ultra-low levels of DNA changes by drinking water: epidemiologically important finding.
,J Biochem.,157 (2) :113-119 2014
Kumari P, Kamiseki M, Biyani M, Suzuki M, Nemoto N, Aita T, Nishigaki K. -
Increasing the library size in cDNA display by optimizing purification procedures.
,Biol Proced Online,15:7 2013
Mochizuki Y, Kumachi S, Nishigaki K, Nemoto N. -
Characterization of Norovirus RNA replicase for in vitro amplification of RNA.
,BMC Biotechnol.,13:85 2013
Arai H, Nishigaki K, Nemoto N, Suzuki M, Husimi Y. -
cDNA displayによる分子デザイン －mRNA display(in vitro virus)からcDNA displayへ－
,生物物理,53:250-253 2013
根本直人、望月佑樹、上野真吾 -
A pull-down method with a biotinylated bait protein prepared by cell-free translation using a puromycin-linker
,Analytical Biochemistry,434:93-95 2013
Yuki Mochizuki, Fumiaki Kohno, Koichi Nishigaki, Naoto Nemoto -
RNAワールドからいかにしてRNPワールドは生じたか
,Viva Origino,41(Supplement):50 2013
根本直人、熊地重文、伏見譲 -
Antagonistic effect of disulfide-rich peptide aptamers selected by cDNA display on interleukin-6-dependent cell proliferation
,Biochemical and Biophysical Research Communications,421:129-133 2012
Naoto Nemoto, Chihiro Tsutsui, Junichi Yamaguchi, Shingo Ueno, Masayuki Machida, Toshikatsu Kobayashi, Takafumi Sakai -
In vitro selection of a novel peptide antagonist of growth hormone secretagogue receptor using cDNA display
,Proc Natl Acad Sci U S A.,109:11121-11126 2012
Shingo Ueno, Sayaka Yoshida, Anupom MONDAL, Kazuya Nishina, Makoto Koyama, Ichiro Sakata, Kenju Miura, Yujiro Hayashi, Naoto Nemoto, Koichi Nishigaki, and Takafumi Sakai -
Photoassisted Recovery of DNA Molecules for On-chip Directed Evolution
,J. Photopolym. Sci. Technol.,25(1):67-72 2012
S. Ueno, A. Ono, R. Kobayashi, Y. Tanaka, S. Sato, M. Biyani, N. Nemoto, and T. Ichiki -
Improvement of a puromycin-linker to extend the selection target varieties in cDNA display method.
, J Biotechnol.,162:299–302 2012 -
cDNA Display: Rapid Stabilization of mRNA Display
,Methods in Mol. Biol.,805:113-135 2012
Shingo Ueno and Naoto Nemoto -
Generation of Functional Peptides Consisting of Four Types of Amino Acids by a cDNA Display Method.
,Protein Science,21(Suppl 1):86-87 2012
S. Kumachi, M. Suzuki, K. Nishigaki,Y. Husimi and N. Nemoto -
Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
,Methods in Mol. Biol.,805:237-250 2012
Yuki Mochizuki and Naoto Nemoto -
In Vitro Evolution of an Enzyme by cDNA Display Method.
,Protein Science,21(Suppl 1):183 2012
N. Nemoto and S. Ueno. -
cDNA Display: Rapid Stabilization of mRNA Display
,Methods in Mol. Biol.,805:113-135 2012
S. Ueno and N. Nemoto -
Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
,Methods in Mol. Biol.,805:237-250 2012
Y. Mochizuki and N. Nemoto -
A function of the primitive protein consisting of four kinds of amino acids
,Viva Origino,40 (Sup):47 2012
S. Kumachi, M. Suzuki, K. Nishigaki, Y. Husimi, N. Nemoto -
An approach towards the origin of primitive translation system from the viewpoint of genotype-phenotype linking strategy
,Viva Origino,40 (Sup):48 2012
N. Nemoto, Y. Husimi -
Directed evolution of a three-finger neurotoxin using cDNA display yields antagonists as well as agonists of interleukin-6 receptor signaling
,Molecular Brain,4:2 2011
Naimuddin, M. , Kobayashi, S. , Tsutsui, C., Machida, M. , Nemoto, N. , Sakai, T. and Kubo, T. -
Novel High-Affinity Aβ-Binding Peptides Identified by an Advanced In vitro
,Protein Pept Lett.,18(6):642-650 2011
Tsuji-Ueno S, Komatsu M, Iguchi K, Takahashi M, Yoshino S, Suzuki M, Nemoto N, Nishigaki K. -
Microintaglio printing of biomolecules and its application to in situ production of messenger ribonucleic acid display microarray
,Applied Physics Express,4(4):47001 2011
M. Biyani, T. Osawa, N. Nemoto, T. Ichiki -
One-pot preparation of mRNA/cDNA display by a novel and versatile puromycin-linker DNA
,ACS Comb. Sci.,13(5):478 485 2011
Y. Mochizuki , M. Biyani , S. Tsuji-Ueno , M. Suzuki , K. Nishigaki , Y. Husimi , and N. Nemoto -
DNA-linked protein array for high-throughput proteomics: from spatially unknown DNA arrays to identifiable protein arrays
,Nano LIFE,1:33-43 2010
Biyani, M., Nemoto, N., Ichiki, T -
Gel shift selection of translation enhancer sequences using messenger RNA display
,Anal. Biochem.,409(1):105-111 2010
Biyani M, Biyani M, Nemoto N, Ichiki T, Nishigaki K, Husimi Y. -
医薬シーズとしての特異的結合性ペプチド（ペプチドアプタマー）の開発
埼玉大学総合研究機構地域共同研究センター産学連携推進部門,埼玉大学地域共同研究センター紀要,8:31-31 2007
西垣 功一, Md. Salimullah, 二上 雅恵, 木下 保則, 内田 秀和, 根本 直人, 門脇 知子, 山本 健二
日本を始め、多くの先進国は超高齢社会を迎えつつあり、老人に多い脳神経疾患の一つであるアルツハイマー病の治療薬が遍く待望されている。そこで、本研究はその原因タンパク質Aβ42 に特異的に結合し、重合を阻害するペプチドの開発を目指した。本年度の研究では先行研究(「地域結集型埼玉バイオプロジェクト」での研究)を受け、Aβ42 に結合するペプチドとして予備的にスクリーニングされたペプチド群の解析を行ない、評価系の開拓を行うとともに、新たな分子ライブラリー（“環状化可能ペプチド”など）を構築し、一層有力な分子をスクリーニングする準備を行った。この際、迅速スクリーニングのために体積活用型マイクロアレイ（ＭＭＶ）の実用化を進め、反応条件・操作法の改良に努meた。 一方、周知の如く、高齢社会では「ガン治療」が、重要な医学テーマである。そこで、先 行研究からガン抑制効果が期待されている「カテプシンＥ“活性向上”ペプチド」の淘汰を すすめ、高い活性を有する数種のペプチドを得た。この際に、ペプチドーペプチド相互作用 を記述するために有効な「タンパク質表面構造のエピトープ様領域への分割・データベース 化」の研究を開始した。 - Presentation
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Searching for Function of Peptides Consisting of Four Kinds of Amino Acids by cDNA Display Method
The Molecular Biology Society of Japan,The 35th Annual Meeting of the Molecular Biology Society of Japan, (Fukuoka, 2012):3P-0762 2012 -
YEAST-BASED LIGAND ASSAY SYSTEM FOR DETECTING G PROTEIN-COUPLED RECEPTOR ACTIVATION IN WATER-IN-OIL DROPLETS
Chemical and Biological Microsystems Society,The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2012):1537-1539 2012
Takashi Sakurai, Ryo Iizuka, Yasuyuki Tanigaki, Rui Sekine, Dong Hyun Yoon, Tetsushi Sekiguchi, Jun Ishii, Akihiko Kondo, Naoto Nemoto, Shuichi Shoji, Takashi Funatsu -
cDNA display法によって得られたIL-6R結合ﾍﾟﾌﾟﾁﾄﾞｱﾌﾟﾀﾏｰのｱﾝﾀｺﾞﾆｽﾄ活性
日本分子生物学会,第33回日本分子生物学会年会(BMB2010):372(3P-1172) 201112
根本直人､筒井千尋､上野真吾､町田雅之､坂井貴文 -
A β 42 結合ペプチドの創製
日本分子生物学会,第33回日本分子生物学会年会(BMB2010):305(2P-1221) 201112
上野-辻幸香､井口翔､小松将之､顧 然､堀田優子､鈴木美穂､根本直人､崎村建司､西垣功一 -
Development of a high-throughput cell-based bioassay for novel bioactive peputides
日本分子生物学会,第33回日本分子生物学会年会(BMB2010):235(1P-1210) 201112
Masayuki Komatsu, Mizuho Sawada, Sachika Tsuji-Ueno, Ran Gu, Koichiro Kitamura, Yuko Hotta, Miho Suzuki, Naoto Nemoto, Kenji Sakimura, Koichi Nishigaki -
Aβ42結合ペプチドの生物活性を測定するｾﾙ/ｳｪﾙ-ﾍﾞｰｽトﾊﾞｲｵｱｯｾｲの開発
日本生物物理学会,50:S162(3P101) 201109
Ran Gu, M.Suzuki, N.Nemoto, K.Nishigaki -
ｼｭｰﾄｹﾞﾉﾑ解析:ｾﾙﾀｲﾌﾟ/ｾﾙﾌｪｰｽﾞの迅速ﾓﾆﾀｰ
日本生物物理学会,50:S162(3P102) 201109
T.Watanabe, M.Suzuki, N.Nemoto, K.Nishigaki -
ペプチド試料の微量迅速機能解析:in vitro translation /MS/gel shift assay
日本生物物理学会,50:S99(2P-098) 201109
M.Komatsu, N.Nemoto, K.Nishigaki -
遺伝子型-表現型対応付け戦略から見た初期翻訳系の起源へのアプロｰチ
日本生物物理学会,50:S70(1P-285) 201109
N.Nemoto, Y.Husimi -
無細胞翻訳系合成タンパク質の迅速な精製技術とそのタンパク質間相互作用解析への応用
日本蛋白質科学会,10:1P-098 201106
福島貴之,小林寿珠子,鈴木美穂,西垣功一,久保泰,根本直人